Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	441,095	T→C	Y343C (TAC→TGC)	yajR ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	441,095	0	T	C	100.0%	124.6 / NA	32	Y343C (TAC→TGC)	yajR	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCG  >  NZ_CP009273/440966‑441230                                                                                                                                   |                                                                                                                                        cTGGCGCCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACAccc                                                                                                                                 <  2:535740/138‑1 (MQ=255)  tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCAt                                                                                                                               >  1:535740/1‑139 (MQ=255)    gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCg                                                                                                                             >  2:464016/1‑139 (MQ=255)         tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTa                                                                                                                        >  1:101848/1‑139 (MQ=255)         tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTa                                                                                                                        >  2:224406/1‑139 (MQ=255)                       cGGCCAGCATTGCGCCACCGAGAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACctg                                                                                                           <  1:275984/138‑1 (MQ=255)                             gctttGCCCCAGCGAGAAATCCCCCCTGCCCGTCAAC‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                                                                                                    <  2:232370/136‑1 (MQ=255)                             gCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                                                                                                    >  2:61516/1‑139 (MQ=255)                             cctttGCCCCAGCGAAAAATCCCCCCTGCCCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                                                                                                    <  2:487200/136‑1 (MQ=255)                               aTTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                                                                                                     >  2:473547/1‑136 (MQ=255)                               aTTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                                                                                                     <  1:473547/136‑1 (MQ=255)                                   cgcCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                                                                                              <  1:614135/139‑1 (MQ=255)                                        gCGAGAAATCCCCCCTGACCGTCAAC‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa                                                                                         <  1:533264/139‑1 (MQ=255)                                        gCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                                                                                                       <  2:9146/125‑1 (MQ=255)                                        gCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                                                                                                       >  1:9146/1‑125 (MQ=255)                                             aaaTCCCCCCGGCCCGTCAAC‑CATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt                                                                                    <  2:455269/139‑1 (MQ=255)                                                  cccccTGACC‑TCAAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAggg                                                                               <  2:240537/139‑1 (MQ=255)                                                      cTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCagg                                                                           <  2:417573/139‑1 (MQ=255)                                                       tGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                                                                                              <  1:100959/119‑1 (MQ=255)                                                       tGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                                                                                              >  2:100959/1‑119 (MQ=255)                                                       tGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCag                                                                            >  1:240537/1‑137 (MQ=255)                                                        gACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCaggag                                                                         >  1:308096/1‑139 (MQ=255)                                                             tCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCtt                                                                    >  2:456878/1‑139 (MQ=255)                                                                      gCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCaaa                                                            >  1:555710/1‑139 (MQ=255)                                                                                  gccgccCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCcac                                                 >  1:516941/1‑138 (MQ=255)                                                                                                 gCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACg                                 <  1:480427/139‑1 (MQ=255)                                                                                                          cacgccaAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGaccac                         >  1:176895/1‑138 (MQ=255)                                                                                                            cgccaAGAAACTGGCTGGTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAg                      >  1:142067/1‑139 (MQ=255)                                                                                                                       tggctggTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGAccacca                       >  2:192984/1‑127 (MQ=255)                                                                                                                       tggctggTGGAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGAccacca                       <  1:192984/127‑1 (MQ=255)                                                                                                                                gAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTgcg  <  1:61516/139‑1 (MQ=255)                                                                                                                                gAGCAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTgcg  <  1:224406/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAA‑CATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGGCAGGAGGGCTTCCATCAAATTAAACGCCACAAAGAAAAGCTGCACGCCGACCACCAGTTGCCAGAACTGCGTTTGCG  >  NZ_CP009273/440966‑441230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑