Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	453,525	T→A	I215N (ATC→AAC)	clpX →	ATP‑dependent protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	453,525	0	T	A	100.0%	99.2 / NA	25	I215N (ATC→AAC)	clpX	ATP‑dependent protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/14);  total (11/14)

CAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCC  >  NZ_CP009273/453401‑453658                                                                                                                             |                                                                                                                                      cAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGt                                                                                                                                <  2:591988/132‑1 (MQ=255) cAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGt                                                                                                                                >  1:591988/1‑132 (MQ=255) cAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTa                                                                                                                          <  1:350630/138‑1 (MQ=255)  aGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAgc                                                                                                                        <  1:228923/139‑1 (MQ=255)          caGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTc                                                                                                                <  1:413741/139‑1 (MQ=255)             cGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCAc                                                                                                             >  1:386801/1‑139 (MQ=255)                            aTCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGt                                                                                               >  2:20225/1‑138 (MQ=255)                            aTCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGAGGGCGt                                                                                               >  2:337761/1‑138 (MQ=255)                                 tGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACAt                                                                                         >  1:629930/1‑139 (MQ=255)                                    aaTCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCg                                                                                      <  1:588187/139‑1 (MQ=255)                                                     gTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTg                                                                       <  1:98348/137‑1 (MQ=255)                                                     gTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTg                                                                       <  2:621075/137‑1 (MQ=255)                                                            aGACAACCCGTCCATTACCCGAGACGTTTCCGGTGTAGGCGTACAGCAGGCACTGTTGAAACTGAACGACGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAATCATCCGCAGCAGGAATTCTTGCAGGTTGAt                                                              <  2:428765/139‑1 (MQ=255)                                                            aGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGa                                                               <  1:206600/138‑1 (MQ=255)                                                               cAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGGAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTg                                                                       >  1:114279/1‑127 (MQ=255)                                                               cAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGGAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTg                                                                       <  2:114279/127‑1 (MQ=255)                                                                       ccATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAgg                                                                                                      <  2:114922/88‑1 (MQ=255)                                                                       ccATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAgg                                                                                                      >  1:114922/1‑88 (MQ=255)                                                                             cccGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGtt                                             >  2:449681/1‑139 (MQ=255)                                                                                            gTGAAGGCGTACAGCAGGCACTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGc                              >  2:4664/1‑139 (MQ=255)                                                                                                                cTGTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAg          <  1:20225/139‑1 (MQ=255)                                                                                                                  gTTGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGt         >  2:589244/1‑138 (MQ=255)                                                                                                                   ttGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGGTAAAGTGa       >  2:370751/1‑139 (MQ=255)                                                                                                                   ttGAAACTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTg        <  1:399407/138‑1 (MQ=255)                                                                                                                        aCTGAACGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTcc  <  2:381498/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                      CAGAAAGCACAGCGTGGTATTGTCTACATCGATGAAATCGACAAGATTTCTCGTAAGTCAGACAACCCGTCCATTACCCGAGACGTTTCCGGTGAAGGCGTACAGCAGGCACTGTTGAAACTGATCGAAGGTACGGTAGCTGCTGTTCCACCGCAAGGTGGGCGTAAACATCCGCAGCAGGAATTCTTGCAGGTTGATACCTCTAAGATCCTGTTTATTTGTGGCGGTGCGTTTGCCGGTCTGGATAAAGTGATTTCC  >  NZ_CP009273/453401‑453658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑