Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	483,263	T→C	D424D (GAT→GAC)	mscK →	mechanosensitive channel MscK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	483,263	0	T	C	100.0%	97.8 / NA	26	D424D (GAT→GAC)	mscK	mechanosensitive channel MscK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/14);  total (12/14)

ACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACC  >  NZ_CP009273/483130‑483396                                                                                                                                      |                                                                                                                                      aCCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGAccaact                                                                                                                                  >  1:445916/1‑139 (MQ=255) aCCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGAccaact                                                                                                                                  >  1:448333/1‑139 (MQ=255)      cagcGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGAccaactcaac                                                                                                                              >  2:621776/1‑138 (MQ=255)         cGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCaacaaac                                                                                                                          <  1:586045/139‑1 (MQ=255)                     ttCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCACCAGCGAAGTTCCCGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCGGGACCAACTCAACAAACAGTTGGGTAccc                                                                                                              >  2:519050/1‑139 (MQ=255)                       ccAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAAc                                                                                                               <  1:122310/136‑1 (MQ=255)                          gagCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAg                                                                                                            >  1:306694/1‑136 (MQ=255)                          gagCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAg                                                                                                            <  2:306694/136‑1 (MQ=255)                               aTGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGAccaactcaac                                                                                                                              <  1:460264/113‑1 (MQ=255)                               aTGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGAccaactcaac                                                                                                                              >  2:460264/1‑113 (MQ=255)                                                   gaagaaGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATc                                                                                 >  1:316297/1‑138 (MQ=255)                                                     agaagGTCACACCAACGAAGTCAACAGCGTAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAAc                                                                              <  2:304432/139‑1 (MQ=255)                                                         ggTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCaaa                                                                                   <  1:573798/130‑1 (MQ=255)                                                         ggTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAAACTGCaaa                                                                                   >  2:573798/1‑130 (MQ=255)                                                           tCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACcagcag                                                                        <  2:128147/139‑1 (MQ=255)                                                           tCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACcagcag                                                                        <  2:621016/139‑1 (MQ=255)                                                           tCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACcagcag                                                                        <  2:448333/139‑1 (MQ=255)                                                                        gTCAACAGCGAAGTTCACGATGCGTTATTGCATGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAg                                                                                                            <  1:587696/90‑1 (MQ=255)                                                                        gTCAACAGCGAAGTTCACGATGCGTTATTGCATGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAg                                                                                                            >  2:587696/1‑90 (MQ=255)                                                                             cAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTc                                                       >  2:507391/1‑138 (MQ=255)                                                                                     ttCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGc                                                                                                           >  1:533708/1‑78 (MQ=255)                                                                                     ttCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGc                                                                                                           <  2:533708/78‑1 (MQ=255)                                                                                               gTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAatccatc                                    >  2:317337/1‑139 (MQ=255)                                                                                                        aGTGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAg                           >  1:158225/1‑139 (MQ=255)                                                                                                          tGGTTGATATGCGTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCa                         <  1:317337/139‑1 (MQ=255)                                                                                                                      gTCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGt             >  1:488082/1‑139 (MQ=255)                                                                                                                       tCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGt             >  1:427741/1‑138 (MQ=255)                                                                                                                       tCGCGAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGt             >  2:626988/1‑138 (MQ=255)                                                                                                                         gcgAATTGCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGaa          <  2:488082/139‑1 (MQ=255)                                                                                                                                gCTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTaa    <  1:386890/138‑1 (MQ=255)                                                                                                                                 cTGGACCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAAcc  <  1:487421/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      ACCAGCAGCGTGATGCACTCTTCCAGAGCGATGCGTTCGTCAACAAACTGGAAGAAGGTCACACCAACGAAGTCAACAGCGAAGTTCACGATGCGTTATTGCAAGTGGTTGATATGCGTCGCGAATTGCTGGATCAACTCAACAAACAGTTGGGTAACCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACC  >  NZ_CP009273/483130‑483396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑