Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	37,396	T→C	A143A (GCA→GCG)	caiC ←	crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	37,396	0	T	C	100.0%	100.5 / NA	29	A143A (GCA→GCG)	caiC	crotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/16);  total (13/16)

GGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCG  >  NZ_CP009273/37282‑37531                                                                                                                   |                                                                                                                                        ggTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGc                                                                                                                 <  1:543294/139‑1 (MQ=255)                  ccgTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGGTTGTTGGTTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATc                                                                                                >  1:228644/1‑139 (MQ=255)                    gTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCtt                                                                                              >  1:82493/1‑139 (MQ=255)                                  tAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGt                                                                                <  1:491091/139‑1 (MQ=255)                                         ggTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa                                                                         <  1:13242/139‑1 (MQ=255)                                         ggTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa                                                                         <  2:438324/139‑1 (MQ=255)                                         ggTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa                                                                         <  1:630935/139‑1 (MQ=255)                                              aTAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGa                                                                    <  2:47174/139‑1 (MQ=255)                                                   acaAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGaa                                                               <  2:182957/139‑1 (MQ=255)                                                     aaGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCa                                                                                                  <  2:573842/102‑1 (MQ=255)                                                     aaGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCa                                                                                                  >  1:573842/1‑102 (MQ=255)                                                     aaGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAAtt                                                             >  2:608879/1‑139 (MQ=255)                                                       ggTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTgc                                                           >  2:591811/1‑139 (MQ=255)                                                       ggTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTgc                                                           >  1:576653/1‑139 (MQ=255)                                                        gTGGCAGGTTGTTGATGTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTgcg                                                          <  2:509575/139‑1 (MQ=255)                                                            cAGGTTGTTGATTTTTCAGTTGCGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACt                                                      >  1:98797/1‑139 (MQ=255)                                                            cAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACt                                                      >  1:391619/1‑139 (MQ=255)                                                            cAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACt                                                      >  1:412629/1‑139 (MQ=255)                                                                   ttgATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCAcc                                               <  2:313865/139‑1 (MQ=255)                                                                   ttgATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCAc                                                >  2:75575/1‑138 (MQ=255)                                                                          ttCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAgg                                         >  1:341876/1‑138 (MQ=255)                                                                                  aGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACact                                                                       <  1:586140/100‑3 (MQ=255)                                                                                  aGTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACact                                                                       >  2:586140/1‑98 (MQ=255)                                                                                            cTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCa                      <  1:362180/139‑1 (MQ=255)                                                                                            cTCACGCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCa                      <  1:504137/139‑1 (MQ=255)                                                                                                 gCCATCATCAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATc                 <  2:576653/139‑1 (MQ=255)                                                                                                    atcatcAGCGGGAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCAc              >  1:104720/1‑139 (MQ=255)                                                                                                              ggAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCt    <  1:16179/139‑1 (MQ=255)                                                                                                                aaGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCg  <  2:104720/139‑1 (MQ=255)                                                                                                                   |                                                                                                                                        GGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGAGTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCG  >  NZ_CP009273/37282‑37531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑