Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 626,664 T→C S439P (TCG→CCG)  cstA → pyruvate/proton symporter CstA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273626,6640TC100.0% 82.7 / NA 23S439P (TCG→CCG) cstApyruvate/proton symporter CstA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/13);  total (10/13)

AACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTG  >  NZ_CP009273/626529‑626792
                                                                                                                                       |                                                                                                                                
aaCAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGa                                                                                                                               >  1:615773/1‑139 (MQ=255)
     cccGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGGGAACAGCCGATCAtt                                                                                                                          >  2:316478/1‑139 (MQ=255)
                                         tgtgGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTgggg                                                                                      <  2:559150/139‑1 (MQ=255)
                                         tgtgGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTgggg                                                                                      <  2:206639/139‑1 (MQ=255)
                                         tgtgGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTgggg                                                                                      <  2:111489/139‑1 (MQ=255)
                                                gCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCt                                                                               <  1:22946/139‑1 (MQ=255)
                                                 cTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTa                                                                              >  2:197315/1‑139 (MQ=255)
                                                                      gTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTggg                                                         <  1:519185/139‑1 (MQ=255)
                                                                               gCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCatgat                                                >  2:347884/1‑139 (MQ=255)
                                                                                   tAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATgatggat                                            >  1:434693/1‑139 (MQ=255)
                                                                                      cATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGAtgtg                                         <  1:193619/139‑1 (MQ=255)
                                                                                          acacCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCg                                      <  2:616805/138‑1 (MQ=255)
                                                                                                gATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGAt                                                                                    <  1:365511/86‑1 (MQ=255)
                                                                                                gATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGAt                                                                                    >  2:365511/1‑86 (MQ=255)
                                                                                                gATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCt                                                                               <  2:174103/91‑1 (MQ=255)
                                                                                                gATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCt                                                                               >  1:174103/1‑91 (MQ=255)
                                                                                                gATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGAtgtg                                         >  2:229100/1‑129 (MQ=255)
                                                                                                gATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGAtgtg                                         <  1:229100/129‑1 (MQ=255)
                                                                                                     gCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCAc                          >  1:452305/1‑139 (MQ=255)
                                                                                                              gATTGCCAGCGAAGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCAtt                 <  1:253675/139‑1 (MQ=255)
                                                                                                                         aaGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGc      <  2:434693/139‑1 (MQ=255)
                                                                                                                         aaGTGGGTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGc      <  2:452305/139‑1 (MQ=255)
                                                                                                                             gggTGAACAGCCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGGATCACTTCGCCATTTTGTTTGAAGCACTg  >  2:28133/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                
AACAGCCCGATGGCGGTGCTGGCTCCGGCAGGGACGGCGGATGTGGTCGCTTCTGCCGCGCAGGTGGTGAGTAGCTGGGGCTTTAGCATTACACCAGATACGCTAAACCAGATTGCCAGCGAAGTGGGTGAACAGTCGATCATTTCCCGTGCGGGCGGTGCGCCGACGCTGGCGGTGGGGATGGCCTACATTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTG  >  NZ_CP009273/626529‑626792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑