| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 724,034 | A→C | V52G (GTC→GGC) | kdpA ← | potassium‑transporting ATPase subunit KdpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 724,034 | 0 | A | C | 96.3% | 88.6 / ‑3.2 | 27 | V52G (GTC→GGC) | kdpA | potassium‑transporting ATPase subunit KdpA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (12/14); minor base T (0/1); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGT > NZ_CP009273/723901‑724168 | cccTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAgccgcc > 1:127003/1‑138 (MQ=255)cccTGGCAACTGCTGGGGATTAAGCGTCAGATAGTGCTGCCCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAgccgcc > 1:106216/1‑138 (MQ=255) ataaaGCGGCAGATAGGGCTGACCGAGCAACAAAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTAcg < 2:379439/136‑1 (MQ=255) tAGTGCTGACCGAGAAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCg < 2:127003/139‑1 (MQ=255) aCCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTAcg < 2:588194/118‑1 (MQ=255) aCCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTAcg > 1:588194/1‑118 (MQ=255) ccGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCgg > 1:639473/1‑139 (MQ=255) gCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCggg > 1:345944/1‑136 (MQ=255) gCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCggg > 1:374393/1‑136 (MQ=255) gCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCggg > 2:176249/1‑136 (MQ=255) cGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGc < 2:499176/139‑1 (MQ=255) gCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCg < 2:186498/102‑1 (MQ=255) gCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCg > 1:186498/1‑102 (MQ=255) gCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGTCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGccc < 1:198398/139‑1 (MQ=255) aTGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGc < 2:525286/139‑1 (MQ=255) tGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCt < 1:594523/139‑1 (MQ=255) gCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTa > 2:324089/1‑137 (MQ=255) gCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGt < 1:478957/97‑1 (MQ=255) gCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGt > 2:478957/1‑97 (MQ=255) cTTCCAGTTCATCTCACGGTCAGCGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAaccc < 2:106216/138‑3 (MQ=255) aTCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGccc > 1:326018/1‑95 (MQ=255) aTCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGccc < 2:326018/95‑1 (MQ=255) ctcACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGaa > 2:400530/1‑68 (MQ=255) ctcACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGaa < 1:400530/68‑1 (MQ=255) aCGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGc > 2:279433/1‑138 (MQ=255) ggTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGAt < 2:345944/139‑1 (MQ=255) ggTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGAt < 1:176249/139‑1 (MQ=255) agagCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCGGCCCGCTGCCTAAAGGACGCGCCAGc < 1:254391/106‑1 (MQ=255) agagCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGc > 2:254391/1‑106 (MQ=255) gagcCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGt > 1:312675/1‑138 (MQ=255) | CCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGT > NZ_CP009273/723901‑724168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |