Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	741,846	T→C	R152R (CGT→CGC)	nei →	endonuclease VIII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	741,846	0	T	C	100.0%	63.8 / NA	18	R152R (CGT→CGC)	nei	endonuclease VIII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/10);  total (8/10)

GGATATTGAGATGTTGACCCCGGAACAACTGACCACGCATCCGTTTTTACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGA  >  NZ_CP009273/741711‑741977                                                                                                                                        |                                                                                                                                    ggATATTGAGATGTTGCCCCCGGAACAACTGCCCACGCATCCGTTTTAACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCa                                                                                                                                   <  2:472299/138‑1 (MQ=255)             gTTGACCCCGGAACAACTGACCACGCATCCGTTTTTACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTa                                                                                                                      >  2:248121/1‑139 (MQ=255)                gACCCCGGAACAACTGACCACGCATCCGTTTTTACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTg                                                                                                                   >  2:535742/1‑139 (MQ=255)                 aCCCCGGAACAACTGACCACGCATCCGTTTTTACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGc                                                                                                                  >  2:174239/1‑139 (MQ=255)                                aCCACGCATCCGTTTTTACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACTCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTCCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGttt                                                                                                    <  1:67949/138‑1 (MQ=255)                                        tCCGTTTTTACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTggg                                                                                           <  2:422341/139‑1 (MQ=255)                                            tttttACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTg                                                                                       >  1:138123/1‑139 (MQ=255)                                                           ggTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGCCGCCGCGTTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCggg                                                                        <  1:284645/139‑1 (MQ=255)                                                            gTCCCGATGTGCTGGATCCGAATCTGACGCCGGGGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCggg                                                                        <  1:227410/138‑1 (MQ=255)                                                               ccGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTg                                                                      >  1:265021/1‑137 (MQ=255)                                                               ccGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGga                                                                    <  1:174239/139‑1 (MQ=255)                                                                     tgCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGGAGATCct                                                              <  2:80811/139‑1 (MQ=255)                                                                                          cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTgg                                         <  1:248121/139‑1 (MQ=255)                                                                                                            gATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAg                                                                                                          <  2:111861/56‑1 (MQ=255)                                                                                                            gATTATTGTCGCCGCGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAg                                                                                                          >  1:111861/1‑56 (MQ=255)                                                                                                                      gccgcGCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGGTGACTGGAAATCATAAAGCGAAAGATCTCAATgcg             >  2:595557/1‑139 (MQ=255)                                                                                                                        cgcgCTTTCGTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATgcggc           >  2:61081/1‑139 (MQ=255)                                                                                                                                 gTAACCGCCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGa  <  2:265021/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GGATATTGAGATGTTGACCCCGGAACAACTGACCACGCATCCGTTTTTACAACGCGTTGGTCCCGATGTGCTGGATCCGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTACTGCTCGATCAGGCGTTTCTGGCTGGGCTTGGCAATTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGA  >  NZ_CP009273/741711‑741977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑