Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 37,524 T→G S101R (AGC→CGC)  caiC ← crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927337,5240TG100.0% 132.2 / NA 36S101R (AGC→CGC) caiCcrotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/14);  total (22/14)

GGAAGTGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCA  >  NZ_CP009273/37391‑37655
                                                                                                                                     |                                                                                                                                   
ggAAGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCt                                                                                                                                <  1:16179/139‑1 (MQ=255)
  aaGCGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCg                                                                                                                              <  2:104720/139‑1 (MQ=255)
                    gCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGtt                                                                                                             >  2:151267/1‑138 (MQ=255)
                       aaTGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         <  1:492934/139‑1 (MQ=255)
                              cGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCa                                                                                                  >  1:615587/1‑139 (MQ=255)
                                  aTTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAAt                                                                                              >  2:614843/1‑139 (MQ=255)
                                          gCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCaa                                                                                      >  2:464528/1‑139 (MQ=255)
                                             tCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAAttt                                                                                   >  2:167487/1‑139 (MQ=255)
                                                  ctgctgAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGc                                                                               <  2:412629/138‑1 (MQ=255)
                                                          tCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGaa                                                                      >  2:248440/1‑139 (MQ=255)
                                                                      tAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGa                                                           >  1:91623/1‑138 (MQ=255)
                                                                      tAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGAt                                                          <  1:151267/139‑1 (MQ=255)
                                                                             gAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTcc                                                   >  2:7770/1‑139 (MQ=255)
                                                                               aTTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGTCAGCCCGAACCAGCAAAAGATa                                                         >  1:357550/1‑131 (MQ=255)
                                                                               aTTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGTCAGCCCGAACCAGCAAAAGATa                                                         <  2:357550/131‑1 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCacaa                                                                                                                         <  2:585150/63‑1 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCacaa                                                                                                                         >  1:585150/1‑63 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         >  1:422118/1‑79 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         <  2:422118/79‑1 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATTAATTCCGGGCAg                                             >  2:141404/1‑139 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  1:134058/1‑139 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  2:108083/1‑139 (MQ=255)
                                                                                   cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  1:158690/1‑139 (MQ=255)
                                                                                     cACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGt                                            >  1:593836/1‑138 (MQ=255)
                                                                                     cACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGt                                            <  2:593836/138‑1 (MQ=255)
                                                                                        tGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTc                                        >  2:400687/1‑139 (MQ=255)
                                                                                                    ggCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCa                                              >  1:492539/1‑121 (MQ=255)
                                                                                                    ggCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCAGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCa                                              <  2:492539/121‑1 (MQ=255)
                                                                                                     gCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCggg                                                >  1:623544/1‑118 (MQ=255)
                                                                                                     gCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCggg                                                <  2:623544/118‑1 (MQ=255)
                                                                                                     gCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGc                            >  2:573014/1‑138 (MQ=255)
                                                                                                     gCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGc                            >  1:41176/1‑138 (MQ=255)
                                                                                                                     tGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         >  2:414707/1‑45 (MQ=255)
                                                                                                                     tGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         <  1:414707/45‑1 (MQ=255)
                                                                                                                     tGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTg            <  2:615587/138‑1 (MQ=255)
                                                                                                                              cACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCa  <  1:141404/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GGAAGTGCCACATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCA  >  NZ_CP009273/37391‑37655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑