Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	780,389	A→G	intergenic (‑110/+4)	zitB ← / ← ybgS	CDF family zinc transporter ZitB/YbgS‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	780,389	0	A	G	100.0%	118.7 / NA	33	intergenic (‑110/+4)	zitB/ybgS	CDF family zinc transporter ZitB/YbgS‑like family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACA  >  NZ_CP009273/780257‑780493                                                                                                                                     |                                                                                                         gtgtgtGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTa                                                                                                    <  2:361577/139‑1 (MQ=255) gtgtgtGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTa                                                                                                    >  1:250039/1‑139 (MQ=255)  tgtgtgAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTAc                                                                                                   >  2:400767/1‑139 (MQ=255)    tgtgagtgtgagTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACt                                                                                                  <  1:619645/138‑1 (MQ=255)        agtgtgagtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGt                                                                                              <  1:193840/138‑1 (MQ=255)              agtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCg                                                                                       >  1:183694/1‑139 (MQ=255)              agtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCg                                                                                       >  1:515659/1‑139 (MQ=255)              agtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCg                                                                                       <  1:400767/139‑1 (MQ=255)               gtgCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGt                                                                                      <  2:179228/139‑1 (MQ=255)                  cgcCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCgg                                                                                   >  1:321727/1‑139 (MQ=255)                   gcCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGt                                                                                  >  2:588850/1‑139 (MQ=255)                             gTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATc                                                                         >  2:535439/1‑138 (MQ=255)                                 tCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGAcat                                                                    <  1:139358/139‑1 (MQ=255)                                                 aCTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCAt                                                     >  1:429940/1‑138 (MQ=255)                                                  cTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCa                                                   >  1:51285/1‑139 (MQ=255)                                                     aaCTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCAcc                                                 <  2:566493/138‑1 (MQ=255)                                                            tGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTa                                         <  2:51285/139‑1 (MQ=255)                                                                 tcatACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCttt                                    <  2:429940/139‑1 (MQ=255)                                                                  catACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCtttt                                   >  2:405654/1‑139 (MQ=255)                                                                                aaGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGa                     <  2:515659/139‑1 (MQ=255)                                                                                   tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAg                  >  2:383457/1‑139 (MQ=255)                                                                                   tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAg                  >  1:71231/1‑139 (MQ=255)                                                                                   tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAg                  >  2:2200/1‑139 (MQ=255)                                                                                   tGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAg                  >  1:606299/1‑139 (MQ=255)                                                                                     aaaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTa                                                                                                    <  2:385992/55‑1 (MQ=255)                                                                                     aaaaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTa                                                                                                    >  1:385992/1‑55 (MQ=255)                                                                                       aaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc              <  1:535439/139‑1 (MQ=255)                                                                                       aaaTTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc              <  1:383457/139‑1 (MQ=255)                                                                                                   agagaCTCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGcaca  <  1:2200/139‑1 (MQ=255)                                                                                                         tCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc              <  2:30799/121‑1 (MQ=255)                                                                                                         tCCGCTCTCCCATTATTGGCTATTTTGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGAc              >  1:30799/1‑121 (MQ=255)                                                                                                                                  tGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTg                                           <  1:91252/67‑1 (MQ=255)                                                                                                                                  tGCGGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTg                                           >  2:91252/1‑67 (MQ=255)                                                                                                                                     |                                                                                                         GTGTGTGAGTGTGAGTGCGCCATGACGAGTCATCCCTTAATTATTCTCACTTAACTTTATGACATCATACTGCTTTTAGAAGTGAAAAATTAAAAGGGAGAGACTCCGCTCTCCCATTATTGGCTATTTTGCAGGGTTACTGCGTGGTACCGTCGGTTTTGGTATCGACATCATTATTGATGCCATCACCGGTTTGTACCTTTTTATTGATATCCGGACAGCGACCATCTTTGCACA  >  NZ_CP009273/780257‑780493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑