Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	796,792	A→G	D193G (GAT→GGT)	ybhH →	4‑oxalomesaconate tautomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	796,792	0	A	G	100.0%	87.5 / NA	24	D193G (GAT→GGT)	ybhH	4‑oxalomesaconate tautomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/12);  total (12/12)

AATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTA  >  NZ_CP009273/796658‑796920                                                                                                                                       |                                                                                                                                 aaTTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATg                                                                                                                              >  2:538341/1‑139 (MQ=255)                      tGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCattatt                                                                                                         >  1:351327/1‑139 (MQ=255)                        ccGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTc                                                                                                        <  1:608523/138‑1 (MQ=255)                         cGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCg                                                                                                      >  1:60343/1‑139 (MQ=255)                                             tCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGaaaaa                                                                                   <  2:60343/138‑1 (MQ=255)                                             tCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGaaaaa                                                                                   >  1:521411/1‑138 (MQ=255)                                                  aaTGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAgg                                                                               <  1:492133/137‑1 (MQ=255)                                                     gCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTAt                                                                           >  1:529387/1‑138 (MQ=255)                                                       cGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGaa                                                                        <  1:538341/139‑1 (MQ=255)                                                       cGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGaa                                                                        >  2:469248/1‑139 (MQ=255)                                                       cGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGaa                                                                        >  2:236812/1‑139 (MQ=255)                                                              aCCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACcgg                                                                 <  2:521411/139‑1 (MQ=255)                                                                   aaCCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTg                                                                                          >  2:145668/1‑109 (MQ=255)                                                                   aaCCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTg                                                                                          <  1:145668/109‑1 (MQ=255)                                                                                ttCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGAc                                                <  2:547663/138‑1 (MQ=255)                                                                                              tCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGcc                                 <  2:489651/139‑1 (MQ=255)                                                                                                  attgattATTTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCa                             >  1:571930/1‑139 (MQ=255)                                                                                                      attattTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTg                          >  2:249029/1‑138 (MQ=255)                                                                                                      attattTTGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTg                          >  1:557832/1‑138 (MQ=255)                                                                                                             tGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTAt                   <  2:463353/138‑1 (MQ=255)                                                                                                             tGACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTAt                   <  1:193455/138‑1 (MQ=255)                                                                                                              gACGATGTCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATc                  <  1:236812/138‑1 (MQ=255)                                                                                                                     tCCCGGTGACCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTAc           >  2:577309/1‑138 (MQ=255)                                                                                                                             aCCTGTATCGGTATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTa  <  2:254112/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 AATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTA  >  NZ_CP009273/796658‑796920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑