Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,015,913	T→G	T155P (ACG→CCG)	sulA ←	cell division inhibitor SulA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,015,913	0	T	G	100.0%	39.9 / NA	12	T155P (ACG→CCG)	sulA	cell division inhibitor SulA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/6);  total (6/6)

ATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCAC  >  NZ_CP009273/1015801‑1016033                                                                                                                 |                                                                                                                         aTTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGAc                                                                                                <  1:592417/139‑1 (MQ=255)               aaaGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAAccc                                                                                   >  1:490735/1‑138 (MQ=255)                                                     aTCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAg                                             >  2:214245/1‑138 (MQ=255)                                                                         tACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAat                        <  2:287844/139‑1 (MQ=255)                                                                              aTTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCt                   >  1:248740/1‑139 (MQ=255)                                                                                   agTGAATTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGccaa              >  1:306293/1‑139 (MQ=255)                                                                                        aTTTTTAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCcaaccaac         >  2:582432/1‑139 (MQ=255)                                                                                         tttttAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCaaccaacc        <  2:7527/139‑1 (MQ=255)                                                                                             tAGCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGAt     <  2:248740/138‑1 (MQ=255)                                                                                               gCCCGGAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATcac  <  1:409003/139‑1 (MQ=255)                                                                                                    gAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGccaacc            >  1:63175/1‑124 (MQ=255)                                                                                                    gAAAGTTGTCTCGGGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGccaacc            <  2:63175/124‑1 (MQ=255)                                                                                                                 |                                                                                                                         ATTAGTCACGACTGAAAGCATTGGCTGGGCGACAAAAAAAGTTCCAGGATTAATCCTAAATTTACTTAATGATACAAATTAGAGTGAATTTTTAGCCCGGAAAGTTGTCTCGTGGCGTGAGAGGATGCGCTTACCGGACGCATAATAAACCCCATAGCGTTACCTTCATTTGCCGCATCAACAAGTTCAGCATGCTCTTCTTCAGTCAAATCATCTGCCAACCAACCGATCAC  >  NZ_CP009273/1015801‑1016033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑