Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,036,585 A→G G171G (GGA→GGG)  appA → bifunctional acid phosphatase/4‑phytase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,036,5850AG96.9% 112.4 / ‑2.8 32coding (513/1299 nt)appAbifunctional acid phosphatase/4‑phytase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (16/15);  minor base . (1/0);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

TGACTGTGCAATAACCGTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCA  >  NZ_CP009273/1036456‑1036717
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tGACTGTGCAATAACCGTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAAtt                                                                                                                             >  1:170707/1‑139 (MQ=255)
     gtgCAATAACCGTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGa                                                                                                                        >  1:521766/1‑139 (MQ=255)
      tgCAATAACCGTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGa                                                                                                                        <  2:151546/138‑1 (MQ=255)
      tgCAATAACCGTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGAc                                                                                                                       >  1:307533/1‑139 (MQ=255)
        cAATAACCGTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACt                                                                                                                      <  1:192615/138‑1 (MQ=255)
                gTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCggg                                                                                                              >  1:347214/1‑138 (MQ=255)
                 tACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCa                                                                                                            >  1:163052/1‑139 (MQ=255)
                           ggCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAG‑‑GGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGc                                                                                                >  2:539147/1‑139 (MQ=255)
                            gCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACgg                                                                                                 >  1:79678/1‑139 (MQ=255)
                                aTACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGtt                                                                                             >  1:492066/1‑139 (MQ=255)
                                   cGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTc                                                                                           <  2:79678/138‑1 (MQ=255)
                                      ccAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTcgcg                                                                                        >  2:224487/1‑138 (MQ=255)
                                                tCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACgg                                                                             <  2:365914/139‑1 (MQ=255)
                                                    ttATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGaa                                                                                <  2:305889/132‑1 (MQ=255)
                                                    ttATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGaa                                                                                >  1:305889/1‑132 (MQ=255)
                                                            tCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAAtttt                                                                 >  1:408637/1‑139 (MQ=255)
                                                            tCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAAtttt                                                                 >  1:600222/1‑139 (MQ=255)
                                                             ccTCTAAAATCTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTc                                                                <  2:525620/139‑1 (MQ=255)
                                                                   aaaaCTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAAt                                                          <  1:224487/139‑1 (MQ=255)
                                                                          gCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACtt                                                   >  1:58770/1‑139 (MQ=255)
                                                                           cGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTtg                                                  <  2:163052/139‑1 (MQ=255)
                                                                                        gATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTg                                     <  1:513668/139‑1 (MQ=255)
                                                                                             cgcgAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGaaa                                >  1:147088/1‑139 (MQ=255)
                                                                                              gcgAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAAc                               <  2:147088/139‑1 (MQ=255)
                                                                                              gcgAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAAc                               <  2:492066/139‑1 (MQ=255)
                                                                                              gcgAACGTGACTGACGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAAc                               <  1:109834/139‑1 (MQ=255)
                                                                                                       actgacGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGa                        >  2:51268/1‑137 (MQ=255)
                                                                                                       actgacGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGa                        >  2:62721/1‑137 (MQ=255)
                                                                                                        ctgacGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAg                     <  2:170707/139‑1 (MQ=255)
                                                                                                        ctgacGCGATCCTCAGCAGGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAg                     <  2:347214/139‑1 (MQ=255)
                                                                                                                      agcagGGCAGGGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGc       <  2:58770/139‑1 (MQ=255)
                                                                                                                           ggcaggGGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCa  >  1:57108/1‑139 (MQ=255)
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TGACTGTGCAATAACCGTACATACCCAGGCAGATACGTCCAGTCCCGATCCGTTATTTAATCCTCTAAAAACTGGCGTTTGCCAACTGGATAACGCGAACGTGACTGACGCGATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAGGACGAAAGCTGTTCATTAACGCAGGCA  >  NZ_CP009273/1036456‑1036717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑