Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,071,087	A→C	G1084G (GGT→GGG)	putA ←	trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,071,087	0	A	C	100.0%	67.9 / NA	19	G1084G (GGT→GGG)	putA	trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/10);  total (9/10)

CTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAG  >  NZ_CP009273/1070955‑1071211                                                                                                                                     |                                                                                                                             cTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACcccaacc                                                                                                                        <  1:399640/139‑1 (MQ=255)            gACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCccac                                                                                                             >  2:65081/1‑139 (MQ=255)            gACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCccac                                                                                                             >  2:17795/1‑139 (MQ=255)                  cgAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCatat                                                                                                        >  1:198823/1‑138 (MQ=255)                                      cTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTAc                                                                                   <  1:301379/139‑1 (MQ=255)                                           cGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAAc                                                                               <  1:264561/138‑1 (MQ=255)                                                  ttCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCg                                                                       <  1:17795/139‑1 (MQ=255)                                                  ttCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCg                                                                       <  1:65081/139‑1 (MQ=255)                                                  ttCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCg                                                                       <  1:483476/139‑1 (MQ=255)                                                              cGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCt                                                           <  1:467107/139‑1 (MQ=255)                                                                           gCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGttt                                               >  2:299904/1‑138 (MQ=255)                                                                           gCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGttt                                               >  2:604058/1‑138 (MQ=255)                                                                             ggACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTtcat                                            <  1:299904/139‑1 (MQ=255)                                                                                   ccTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATgc                                      >  1:339288/1‑139 (MQ=255)                                                                                                cGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGcc                         <  1:202726/139‑1 (MQ=255)                                                                                                      cGGACAACCCTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGt                   <  2:469364/139‑1 (MQ=255)                                                                                                             cccTTCGCCGCCGAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCAGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCa            >  2:376472/1‑139 (MQ=255)                                                                                                                       ccgAACGGCTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAg  >  1:118423/1‑139 (MQ=255)                                                                                                                               cTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCa                        <  1:422244/109‑1 (MQ=255)                                                                                                                               cTGCACCCCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCa                        >  2:422244/1‑109 (MQ=255)                                                                                                                                     |                                                                                                                             CTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAG  >  NZ_CP009273/1070955‑1071211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑