Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,083,520	A→G	P598P (CCT→CCC)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase PgaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,083,520	0	A	G	100.0%	48.3 / NA	14	P598P (CCT→CCC)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase PgaB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/9);  total (5/9)

ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTG  >  NZ_CP009273/1083388‑1083653                                                                                                                                     |                                                                                                                                      aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGgggg                                                                                                                                    >  1:11527/1‑136 (MQ=255) aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGgggg                                                                                                                                    <  2:11527/136‑1 (MQ=255)                         tAATAGGCCCATCCAGTGAGCGAGTTGTTGCGAAGCACTAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGCTTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTg                                                                                                          >  2:392333/1‑138 (MQ=255)                                                            aaaTAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACAcc                                                                      >  2:476452/1‑139 (MQ=255)                                                              aTAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCtt                                                                    <  2:598984/139‑1 (MQ=255)                                                               tAGCCTGATGCTGACCATTGATCTGCCCGTTGTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTc                                                                   <  1:186812/139‑1 (MQ=255)                                                                      aTGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGAt                                                              >  1:53545/1‑137 (MQ=255)                                                                         cTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGc                                                         <  2:351932/139‑1 (MQ=255)                                                                            aCCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCa                                                        >  1:606035/1‑137 (MQ=255)                                                                            aCCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCAt                                                       <  2:53545/138‑1 (MQ=255)                                                                                                   ccTGTAATTCTAAAATAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCAt                                <  2:393952/138‑1 (MQ=255)                                                                                                                 aTAGATTTGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAAt                 <  2:307989/139‑1 (MQ=255)                                                                                                                      tttGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCCTTTTAGGAAATCAGCa            <  1:392333/139‑1 (MQ=255)                                                                                                                       ttGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGc                                                         >  1:521890/1‑93 (MQ=255)                                                                                                                       ttGTCTTTAGCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGc                                                         <  2:521890/93‑1 (MQ=255)                                                                                                                                gCCTGGGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCtgtg  <  1:476452/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTG  >  NZ_CP009273/1083388‑1083653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑