Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,194,575	A→C	D142A (GAC→GCC)	lit →	cell death peptidase Lit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,194,575	0	A	C	100.0%	66.4 / NA	19	D142A (GAC→GCC)	lit	cell death peptidase Lit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/10);  total (9/10)

AAAAGTCTGATGAACTTCTTAAATGGGCAAGGAAAAACTTGCAAACAACAGGTTGCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACA  >  NZ_CP009273/1194464‑1194710                                                                                                                |                                                                                                                                        aaaaGTCTGATGAACTTCTTAAATGGGCAAGGAAAAACTTGCAAACAACAGGTTGCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCt                                                                                                              <  2:542980/139‑1 (MQ=255)    aGTCTGATGAACTTCTTAAATGGGCAAGGAAAAACTTGCAAACAACAGGTTGCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTtg                                                                                                           <  2:554503/139‑1 (MQ=255)                                     aCTTGCAAACAACAGGTTGCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAg                                                                          >  2:397861/1‑139 (MQ=255)                                                      tGCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAg                                                          >  1:580816/1‑138 (MQ=255)                                                       gCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGttt                                                               >  1:261302/1‑132 (MQ=255)                                                       gCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGttt                                                               <  2:261302/132‑1 (MQ=255)                                                         gAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGata                                                                                                                   <  2:93292/78‑1 (MQ=255)                                                         gAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGata                                                                                                                   >  1:93292/1‑78 (MQ=255)                                                                   cTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCAc                                            <  2:580816/139‑1 (MQ=255)                                                                               ccAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATtctc                                <  1:104615/139‑1 (MQ=255)                                                                               ccAAGCCAGAAGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATtcac                                <  1:74981/139‑3 (MQ=255)                                                                                         aGCATATTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAagag                      <  2:454987/139‑1 (MQ=255)                                                                                             tatTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCAtg                                                                     <  1:132442/88‑1 (MQ=255)                                                                                             tatTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCAtg                                                                     >  2:132442/1‑88 (MQ=255)                                                                                             tatTTACAAGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGc                  >  2:554558/1‑139 (MQ=255)                                                                                                     aGGAAGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAg            >  1:122443/1‑137 (MQ=255)                                                                                                         aGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTTTTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGAtt      >  1:462143/1‑139 (MQ=255)                                                                                                         aGCGAAGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGAtt      >  2:108241/1‑139 (MQ=255)                                                                                                             aaGCCTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTcaca  <  1:309511/139‑1 (MQ=255)                                                                                                                |                                                                                                                                        AAAAGTCTGATGAACTTCTTAAATGGGCAAGGAAAAACTTGCAAACAACAGGTTGCGAATCATGGCCTAAAAAATGTCCCAAGCCAGAAGCATATTTACAAGGAAGCGAAGACTCACAAGTTGCTAGCGAGATATTTCTTTGTGCTATTGCTTGGATTCTTCATCATGAAATAAGTCATGTTGTTTTACAGCATCCATTGGTCACTACAGCATTCTCCACTCAAGAGGAGCGTGAAGCAGATTCACA  >  NZ_CP009273/1194464‑1194710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑