| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,312,468 | T→C | T69A (ACG→GCG) | trpB ← | tryptophan synthase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,312,468 | 0 | T | C | 100.0% | 41.9 / NA | 13 | T69A (ACG→GCG) | trpB | tryptophan synthase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/5); total (8/5) | |||||||||||
TGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCG > NZ_CP009273/1312354‑1312597 | tgatTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGtt < 1:5946/130‑1 (MQ=255)tgatTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGtt > 2:5946/1‑130 (MQ=255) aCCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAgcg < 1:83815/134‑1 (MQ=255) aCCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAgcg > 2:83815/1‑134 (MQ=255) ccccAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCCTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCTCGGCATAGTTTTTcagca < 1:366877/137‑1 (MQ=255) tGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAAtt > 2:294147/1‑139 (MQ=255) caAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCa < 1:294147/139‑1 (MQ=255) agaTACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGCACTGAGCCTGAAATTCAGGCTCTTTTTGCGCACTGACACAAGcttctt > 1:140271/1‑139 (MQ=255) aGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCt > 2:445744/1‑139 (MQ=255) gCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTg > 1:501895/1‑139 (MQ=255) ggtgTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcg > 2:486318/1‑138 (MQ=255) gtgtTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCAt < 1:289442/58‑1 (MQ=255) gtgtTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCAt > 2:289442/1‑58 (MQ=255) | TGATTTCGGTTTTACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCG > NZ_CP009273/1312354‑1312597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |