Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,386,575	T→C	L110L (TTA→CTA)	pgrR →	DNA‑binding transcriptional regulator PgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,386,575	0	T	C	100.0%	97.2 / NA	26	L110L (TTA→CTA)	pgrR	DNA‑binding transcriptional regulator PgrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/10);  total (16/10)

CATGACATAGATTCAGCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCTGGGTGGG  >  NZ_CP009273/1386473‑1386694                                                                                                       |                                                                                                                        cATGACATAGATTCAGCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCt                                                                                      <  2:473183/138‑1 (MQ=255)      cATAGATTCAGCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAAtt                                                                                >  1:384474/1‑139 (MQ=255)            ttCAGCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATAtt                                                                          <  1:375486/139‑1 (MQ=255)                gCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGc                                                                      >  2:579406/1‑139 (MQ=255)                 ccATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCt                                                                     <  1:349659/139‑1 (MQ=255)                 ccATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCt                                                                     <  2:33369/139‑1 (MQ=255)                     ggCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCAc                                                                  >  1:88660/1‑138 (MQ=255)                               aGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATg                                                       >  2:249745/1‑139 (MQ=255)                                    tCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt                                                   >  2:61859/1‑138 (MQ=255)                                    tCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGttt                                                   >  2:119521/1‑138 (MQ=255)                                              cGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATACGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCg                                        >  1:585141/1‑139 (MQ=255)                                                cccATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGtt                                      >  1:431197/1‑139 (MQ=255)                                                              tACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGa                        >  2:39032/1‑139 (MQ=255)                                                                 gTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGc                     <  1:61859/139‑1 (MQ=255)                                                                 gTATTACTACTGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGc                     <  2:520701/139‑1 (MQ=255)                                                                        tactGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGcgtc              >  2:262848/1‑139 (MQ=255)                                                                          ctGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTg                                  >  2:476915/1‑117 (MQ=255)                                                                          ctGTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTg                                  <  1:476915/117‑1 (MQ=255)                                                                            gTAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGcgtccgtc          >  1:11266/1‑139 (MQ=255)                                                                             tAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGcgtccgtc          <  1:579406/138‑1 (MQ=255)                                                                             tAGAACATGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCt         >  1:635057/1‑139 (MQ=255)                                                                                    tGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCtgggtggg  >  2:388494/1‑139 (MQ=255)                                                                                    tGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCt         <  1:205592/132‑1 (MQ=255)                                                                                    tGCAGCAAAAACGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCt         >  2:205592/1‑132 (MQ=255)                                                                                            aaaCGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCtgggtggg  <  1:624421/131‑1 (MQ=255)                                                                                            aaaCGATATTGCTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCtgggtggg  >  2:624421/1‑131 (MQ=255)                                                                                                       |                                                                                                                        CATGACATAGATTCAGCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACTGTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCATCCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGTTTTGATGCAGGCGTCCGTCTGGGTGGG  >  NZ_CP009273/1386473‑1386694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑