Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,412,526	T→G	intergenic (‑40/‑402)	kilR ← / → sieB	killing protein KilR/superinfection exclusion B family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,412,526	0	T	G	100.0%	143.8 / NA	39	intergenic (‑40/‑402)	kilR/sieB	killing protein KilR/superinfection exclusion B family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/16);  total (23/16)

ATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTT  >  NZ_CP009273/1412400‑1412660                                                                                                                               |                                                                                                                                       atatGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTc                                                                                                                            >  2:563241/1‑139 (MQ=255)     gTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAgg                                                                                                                        >  2:492743/1‑139 (MQ=255)      tCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGt                                                                                                                       >  2:627327/1‑139 (MQ=255)        cGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGa                                                                                                                     >  1:417857/1‑139 (MQ=255)          gcgttCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGAGATGTGCAATCATAATTATTTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGTCTCCAGGTGACt                                                                                                                   >  2:186462/4‑139 (MQ=255)          gCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACt                                                                                                                   >  2:73851/1‑139 (MQ=255)               cATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAGCTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCa                                                                                                                <  1:492743/137‑1 (MQ=255)                        cATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAAc                                                                                                      >  2:332442/1‑138 (MQ=255)                                ccAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACa                                                                                               <  2:333462/137‑1 (MQ=255)                                ccAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACa                                                                                               >  1:333462/1‑137 (MQ=255)                                         acacacTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTc                                                                                     <  1:563241/138‑1 (MQ=255)                                             acTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTcccacc                                                                                <  1:627327/139‑1 (MQ=255)                                             acTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTcccacc                                                                                <  1:275273/139‑1 (MQ=255)                                                 aCGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTcccaccca                                                                              <  2:58190/137‑1 (MQ=255)                                                     ggTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGt                                                                                                                                 >  1:122080/1‑82 (MQ=255)                                                     ggTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGt                                                                                                                                 <  2:122080/82‑1 (MQ=255)                                                     ggTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAg                                                                                                                         >  1:424762/1‑90 (MQ=255)                                                     ggTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAg                                                                                                                         <  2:424762/90‑1 (MQ=255)                                                       tATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCaa                                                                                                               >  1:462699/1‑98 (MQ=255)                                                       tATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCaa                                                                                                               <  2:462699/98‑1 (MQ=255)                                                         tttCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAg                                                                    >  1:186474/1‑139 (MQ=255)                                                                 gTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTg                                                                                                                                   >  2:286964/1‑68 (MQ=255)                                                                 gTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTg                                                                                                                                   <  1:286964/68‑1 (MQ=255)                                                                 gTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACa                                                            >  2:274169/1‑139 (MQ=255)                                                                 gTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACa                                                            >  1:588021/1‑139 (MQ=255)                                                                            aTGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGtt                                                                                                         >  2:377400/1‑83 (MQ=255)                                                                            aTGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGtt                                                                                                         <  1:377400/83‑1 (MQ=255)                                                                            aTGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAAc                                                 >  1:158332/1‑139 (MQ=255)                                                                                              ctcCATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAg                               >  1:246652/1‑139 (MQ=255)                                                                                                 cATTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGcc                             <  2:417857/138‑1 (MQ=255)                                                                                                  aTTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAgg                                                                                                                        >  2:411620/1‑46 (MQ=255)                                                                                                  aTTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAgg                                                                                                                        <  1:411620/46‑1 (MQ=255)                                                                                                  aTTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTcccaccca                                                                              >  1:423056/1‑88 (MQ=255)                                                                                                  aTTTTTACAAATGAACTTTGTTGATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTcccaccca                                                                              <  2:423056/88‑1 (MQ=255)                                                                                                                      ttgATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAAtt       <  1:318716/139‑1 (MQ=255)                                                                                                                      ttgATGCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAAtt       >  2:182461/1‑139 (MQ=255)                                                                                                                           gCGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACtt  >  1:316620/1‑139 (MQ=255)                                                                                                                           gCGGGGTCTGGTGCCTCCAGGTGACTGAAACCAGTTAAAAATTACAGTAGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGATTAGCCGCAGTCACCGCATCACAAAATTAACtt  >  1:557567/1‑139 (MQ=255)                                                                                                                            cGGGGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACtt  <  2:232598/138‑1 (MQ=255)                                                                                                                               |                                                                                                                                       ATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTCTGGTGCCTCCAGGTGACTGCAACCAGTTAACAATTACAGTCGGCTTTCCCACCCAAACCAATAAGGACTAACATGACTTTTAACTGTGCCACGTGCGCTTAGCCGCATTCACCGCATCACAAAATTCACTT  >  NZ_CP009273/1412400‑1412660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑