Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,443,877	T→C	D577G (GAC→GGC)	tynA ←	primary‑amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,443,877	0	T	C	100.0%	182.7 / NA	47	D577G (GAC→GGC)	tynA	primary‑amine oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/26);  total (21/26)

TCGGGTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCG  >  NZ_CP009273/1443743‑1444003                                                                                                                                       |                                                                                                                               tCGGGTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCAt                                                                                                                            >  2:51440/1‑139 (MQ=255) tCGGGTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCAt                                                                                                                            >  2:119157/1‑139 (MQ=255) tCGGGTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCAt                                                                                                                            >  1:569245/1‑139 (MQ=255) tCGGGTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCAt                                                                                                                            >  1:505652/1‑139 (MQ=255)  cGGGTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCAt                                                                                                                            <  1:608949/138‑1 (MQ=255)     gTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGc                                                                                                                         >  1:445479/1‑138 (MQ=255)                   gAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTAtt                                                                                                          <  1:437662/139‑1 (MQ=255)                        gTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCg                                                                                                      <  1:127174/138‑1 (MQ=255)                        gTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGc                                                                                                     <  2:638889/139‑1 (MQ=255)                        gTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGc                                                                                                     <  2:331842/139‑1 (MQ=255)                        gTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGc                                                                                                     <  1:462661/139‑1 (MQ=255)                                                           aTTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAata                                                                  <  2:313966/139‑1 (MQ=255)                                                                cGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             >  1:356923/1‑139 (MQ=255)                                                                cGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             <  1:264898/139‑1 (MQ=255)                                                                  aTGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGgt                                                           <  1:316048/139‑1 (MQ=255)                                                                  aTGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGgt                                                           <  1:163857/139‑1 (MQ=255)                                                                  aTGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGgt                                                           <  2:505652/139‑1 (MQ=255)                                                                     ttgtACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGgtgtg                                                        <  1:475529/139‑1 (MQ=255)                                                                        tACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTgta                                                      <  2:113405/138‑1 (MQ=255)                                                                          cTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTgtagt                                                    <  2:445479/138‑1 (MQ=255)                                                                             aTTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATg                                                                >  2:186383/1‑123 (MQ=255)                                                                             aTTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATg                                                                <  1:186383/123‑1 (MQ=255)                                                                             aTTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTAcc                                                 <  2:382355/138‑1 (MQ=255)                                                                             aTTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTAcc                                                 >  1:573373/1‑138 (MQ=255)                                                                             aTTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTAc                                                  <  1:202095/137‑1 (MQ=255)                                                                                       aTGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATtgt                                      >  1:506308/1‑139 (MQ=255)                                                                                       aTGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATtgt                                      >  2:4588/1‑139 (MQ=255)                                                                                                gTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATc                                                                                                 >  2:5667/1‑71 (MQ=255)                                                                                                gTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATc                                                                                                 <  1:5667/71‑1 (MQ=255)                                                                                                gTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGAt                               >  1:14797/1‑137 (MQ=255)                                                                                                   cGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCg                          <  2:476620/139‑1 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             >  2:510025/1‑102 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             >  2:612504/1‑102 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             >  1:134996/1‑102 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             <  2:134996/102‑1 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             <  1:612504/102‑1 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTg                                                             <  1:510025/102‑1 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTg                        >  2:155910/1‑139 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTg                        >  2:113714/1‑139 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTg                        >  1:258884/1‑139 (MQ=255)                                                                                                     tGGGCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTg                        >  1:376698/1‑139 (MQ=255)                                                                                                        gCCACCGGCAGTATTCGGTTTTACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCg                     <  1:4588/139‑1 (MQ=255)                                                                                                                          ttttACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATaa                             <  1:52713/113‑1 (MQ=255)                                                                                                                          ttttACCACGGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATaa                             >  2:52713/1‑113 (MQ=255)                                                                                                                            ttACCACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCg  <  1:28116/138‑1 (MQ=255)                                                                                                                               ccACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATtg                                       >  2:342946/1‑98 (MQ=255)                                                                                                                               ccACTGGGCCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATtg                                       <  1:342946/98‑1 (MQ=255)                                                                                                                                       |                                                                                                                               TCGGGTTACTCAACAGACGAATCGTGCCCGGATCAAATTTCTGTGCGGCATCCTGTTCATTGCCGATGTTGTACTGATTAACTTGCATGGTACTGGTGCGTGGGCCACCGGCAGTATTCGGTTTTACCACTGGGTCCATCGCCACCAGGCTGTTATTCTCGCCATCTACATCCAGATCGAGGCGGAAATTATAAATATGTTGGTGTGTAGTACCCACGATATTGTGATCGATAAGCGTGCCGTAGCGCGTGTCATCTTTCG  >  NZ_CP009273/1443743‑1444003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑