Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,492,952	A→G	E15E (GAA→GAG)	ydcH →	YdcH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,492,952	0	A	G	100.0%	142.8 / NA	39	E15E (GAA→GAG)	ydcH	YdcH family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/17);  total (22/17)

GATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGAT  >  NZ_CP009273/1492818‑1493087                                                                                                                                       |                                                                                                                                        gATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATc                                                                                                                                     <  1:73524/139‑1 (MQ=255)  aTCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATcc                                                                                                                                    >  1:354293/1‑139 (MQ=255)  aTCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATcc                                                                                                                                    >  1:342241/1‑139 (MQ=255)            cATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCt                                                                                                                                   >  2:607805/1‑130 (MQ=255)            cATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCt                                                                                                                                   <  1:607805/130‑1 (MQ=255)                tttCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCt                                                                                                                      >  1:448281/1‑139 (MQ=255)                  tCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTg                                                                                                                    >  2:169570/1‑139 (MQ=255)                            aaaTGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATaaa                                                                                                           <  2:354293/138‑1 (MQ=255)                            aaaTGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATaaa                                                                                                           <  1:88226/138‑1 (MQ=255)                            aaaTGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATaaa                                                                                                           <  1:550726/138‑1 (MQ=255)                            aaaTGAGGAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATaaa                                                                                                           <  2:553034/138‑1 (MQ=255)                             aacgAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCTCTAACAGGGAGTGCTTATGTTTCCAGAATACCGAGATTAAATATCCCGGCTGAAAATCGTGAATCCTCGCTTTATGTCCTTCTTCGATAAac                                                                                                          <  2:466710/135‑1 (MQ=255)                                           tGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCACGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGAt                                                                                            >  1:385952/1‑138 (MQ=255)                                                tgtATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGa                                                                                       >  2:341846/1‑138 (MQ=255)                                                tgtATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGa                                                                                       >  2:108765/1‑138 (MQ=255)                                                      tCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGc                                                                                 <  2:284792/138‑1 (MQ=255)                                                          aTACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGa                                                                             >  1:205736/1‑138 (MQ=255)                                                          aTACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGa                                                                             >  1:481071/1‑138 (MQ=255)                                                                   gTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGtt                                                                   >  1:552663/1‑139 (MQ=255)                                                                       gCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGa                                                               >  2:67883/1‑139 (MQ=255)                                                                           cTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGt                                                           <  2:433091/139‑1 (MQ=255)                                                                                   gAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTa                                                   <  2:77111/139‑1 (MQ=255)                                                                                   gAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTa                                                   <  2:448281/139‑1 (MQ=255)                                                                                   gAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTa                                                   >  1:537978/1‑139 (MQ=255)                                                                                        cGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAaagga                                                                        >  2:271450/1‑113 (MQ=255)                                                                                        cGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAaagga                                                                        <  1:271450/113‑1 (MQ=255)                                                                                                     aaTACCGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCg                                 >  1:297594/1‑139 (MQ=255)                                                                                                          cGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATg                             >  2:134729/1‑138 (MQ=255)                                                                                                          cGAGATTTAATATCCCGTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATg                             >  1:339818/1‑138 (MQ=255)                                                                                                                          gTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATaa                                                                                                            >  2:12422/1‑43 (MQ=255)                                                                                                                          gTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATaa                                                                                                            <  1:12422/43‑1 (MQ=255)                                                                                                                          gTCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACa            <  2:297594/139‑1 (MQ=255)                                                                                                                           tCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTa              <  2:530516/136‑1 (MQ=255)                                                                                                                           tCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTa              >  1:530516/1‑136 (MQ=255)                                                                                                                           tCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAg           >  1:483389/1‑139 (MQ=255)                                                                                                                           tCTGAAAAACGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAg           >  1:505844/1‑139 (MQ=255)                                                                                                                               aaaaaCGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTaa       <  1:52895/139‑1 (MQ=255)                                                                                                                                    cGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAagat  <  1:491670/139‑1 (MQ=255)                                                                                                                                    cGAGAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAagat  >  2:491670/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        GATCCCGCTCGCATTTTTCCCTAAGTTAAATGAGTAATCTGATGGTGTGTATTTCAGATACACCTTGTCAGCCACTAACAGGGAGTGCGTATGTTTCCAGAATACCGAGATTTAATATCCCGTCTGAAAAACGAAAATCCTCGCTTTATGTCCTTGTTCGATAAACACAATAAACTTGATCATGAAATTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGAT  >  NZ_CP009273/1492818‑1493087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑