Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,540,382	T→G	intergenic (‑97/+163)	yddL ← / ← yddG	porin/aromatic amino acid efflux DMT transporter YddG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,540,382	0	T	G	100.0%	26.2 / NA	8	intergenic (‑97/+163)	yddL/yddG	porin/aromatic amino acid efflux DMT transporter YddG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (3/5);  total (3/5)

GTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAAT  >  NZ_CP009273/1540254‑1540480                                                                                                                                 |                                                                                                   gTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTaaa                                                                                             <  1:60474/136‑1 (MQ=255) gTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTaaa                                                                                             >  2:60474/1‑136 (MQ=255)           ccGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTAAAAACAATGAGaaaa                                                                                <  1:218383/139‑1 (MQ=255)           ccGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTAAAAACAATGAGaaa                                                                                 >  2:353474/1‑138 (MQ=255)                               aTTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTAAAAACAATGAGAAAAATAGATGAAATAAtattat                                                             <  2:298914/138‑1 (MQ=255)                                      aGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGat                                                     <  1:626603/139‑1 (MQ=255)                                                      tCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCg                                      <  1:353474/138‑1 (MQ=255)                                                                                         cGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGGGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAat  >  1:455465/1‑139 (MQ=255)                                                                                                                                 |                                                                                                   GTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAAT  >  NZ_CP009273/1540254‑1540480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑