Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,620,474	A→G	I388I (ATT→ATC)	dcp ←	peptidyl‑dipeptidase Dcp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,620,474	0	A	G	100.0%	123.1 / NA	33	I388I (ATT→ATC)	dcp	peptidyl‑dipeptidase Dcp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

TCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCT  >  NZ_CP009273/1620343‑1620574                                                                                                                                    |                                                                                                     tCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCa                                                                                                >  2:282479/1‑138 (MQ=255)            gCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGAc                                                                                     <  1:594808/138‑1 (MQ=255)              ccatccatGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACaaa                                                                                  <  1:247072/139‑1 (MQ=255)                        aCCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCg                                                                        <  1:309918/139‑1 (MQ=255)                        aCCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCg                                                                        <  1:513897/139‑1 (MQ=255)                          cgccgcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGaa                                                                      <  1:282479/139‑1 (MQ=255)                            ccgcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGaa                                                                      >  2:443927/1‑137 (MQ=255)                            ccgcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGaa                                                                      >  1:634032/1‑137 (MQ=255)                            ccgcTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGaa                                                                      >  1:585861/1‑137 (MQ=255)                                          aCGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTc                                                      >  2:604838/1‑139 (MQ=255)                                          aCGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTc                                                      <  2:211166/139‑1 (MQ=255)                                              gCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAg                                                   >  2:348487/1‑138 (MQ=255)                                                 aagaaATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATa                                               <  2:585861/139‑1 (MQ=255)                                                      aTCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAACCTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCt                                          <  2:222677/139‑1 (MQ=255)                                                      aTCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCt                                          <  1:604838/139‑1 (MQ=255)                                                      aTCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCt                                          <  2:376458/139‑1 (MQ=255)                                                      aTCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCt                                          <  1:348487/139‑1 (MQ=255)                                                                     cGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACt                                                                                <  1:546283/86‑1 (MQ=255)                                                                     cGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACt                                                                                >  2:546283/1‑86 (MQ=255)                                                                     cGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCgtg                           >  1:181002/1‑139 (MQ=255)                                                                           ccccACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGAc                                                                                     >  1:634519/1‑75 (MQ=255)                                                                           ccccACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGAc                                                                                     <  2:634519/75‑1 (MQ=255)                                                                           ccccACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAAt                     >  1:519312/1‑139 (MQ=255)                                                                           ccccACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAAt                     >  1:415324/1‑139 (MQ=255)                                                                                      tATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGAc                                                                                     <  1:155692/64‑1 (MQ=255)                                                                                      tATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGAc                                                                                     >  2:155692/1‑64 (MQ=255)                                                                                              aaaaTTTCCCACACACGAACGTCAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCt  <  2:181002/139‑1 (MQ=255)                                                                                                                    cAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTc                                                      <  2:66405/65‑1 (MQ=255)                                                                                                                    cAGGATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTc                                                      >  1:66405/1‑65 (MQ=255)                                                                                                                      ggATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAagag                                                                   <  1:73603/50‑1 (MQ=255)                                                                                                                      ggATGGTAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAagag                                                                   >  2:73603/1‑50 (MQ=255)                                                                                                                            tAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTc                                                      <  2:5625/57‑1 (MQ=255)                                                                                                                            tAGACAGGGATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTc                                                      >  1:5625/1‑57 (MQ=255)                                                                                                                                    |                                                                                                     TCAACAAAATTGCCCATCCATGCACCGCCGCTTTTTGAATCACGGGCGAAGAAATCACCGTAAAATAACGCCAGCCCCACGCCATTATGATCAAAAATTTCCCACACACGAACGTCAGGATGGTAGACAGGAATATCAAAACGTTCGACAAACTTAATACCGAAGAGCTGATTCGCGGTCCAGAATACACCTTCATTTAACACCGTGTTTAATTCAAAATATGGCTTGAGCT  >  NZ_CP009273/1620343‑1620574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑