Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,624,590	T→C	D194G (GAC→GGC)	ydfI ←	mannitol dehydrogenase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,624,590	0	T	C	100.0%	69.1 / NA	21	D194G (GAC→GGC)	ydfI	mannitol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

TTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGAT  >  NZ_CP009273/1624454‑1624719                                                                                                                                         |                                                                                                                                  ttgttCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCa                                                                                                                                 <  1:497015/139‑1 (MQ=255) ttgttCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCa                                                                                                                                 <  1:459999/139‑1 (MQ=255)    ttCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACg                                                                                                                               >  2:7597/1‑138 (MQ=255)                         ctctGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGc                                                                                                         <  1:291519/139‑1 (MQ=255)                                      ggCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCacat                                                                                             >  2:553778/1‑138 (MQ=255)                                        cacaATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCacatga                                                                                           >  1:321656/1‑138 (MQ=255)                                           aaTACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCacatgacatg                                                                                       <  1:193999/139‑1 (MQ=255)                                                                 gggAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCg                                                                                                                  >  1:204538/1‑90 (MQ=255)                                                                 gggAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCg                                                                                                                  <  2:204538/90‑1 (MQ=255)                                                                  ggAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTg                                                                 <  2:464823/138‑1 (MQ=255)                                                                  ggAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTg                                                                 <  2:269657/138‑1 (MQ=255)                                                                      aGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCggg                                                                         >  1:380747/1‑126 (MQ=255)                                                                      aGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCggg                                                                         <  2:380747/126‑1 (MQ=255)                                                                                           ttGTGCCAGTTTTACAGCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTca                                       <  2:128431/139‑1 (MQ=255)                                                                                             gtgCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTcaa                                      <  1:2671/138‑1 (MQ=255)                                                                                                    ttttACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCAcc                              >  1:214783/1‑139 (MQ=255)                                                                                                              aCGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTca                                       <  2:384736/120‑1 (MQ=255)                                                                                                              aCGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTca                                       >  1:384736/1‑120 (MQ=255)                                                                                                                           aGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCtggtg       >  1:590550/1‑139 (MQ=255)                                                                                                                                gTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGAt  >  1:390444/1‑139 (MQ=255)                                                                                                                                gTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGAt  >  1:238447/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                  TTGTTCGATTTTCGCCAGCGTATCCTCTGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGGGGAT  >  NZ_CP009273/1624454‑1624719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑