Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,658,762	A→G	intergenic (+42/‑1)	ynfH → / → dmsD	dimethyl sulfoxide reductase anchor subunit/Tat proofreading chaperone DmsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,658,762	0	A	G	100.0%	159.6 / NA	42	intergenic (+42/‑1)	ynfH/dmsD	dimethyl sulfoxide reductase anchor subunit/Tat proofreading chaperone DmsD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/17);  total (25/17)

AATCCTCATACTTGGCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACT  >  NZ_CP009273/1658636‑1658894                                                                                                                               |                                                                                                                                     aaTCCTCATACTTGGCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCAtttt                                                                                                                          >  1:6461/1‑139 (MQ=255)              ggCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGCTGTTAGGGATGACCCATTTTTCACAGCAAGAt                                                                                                              >  2:204803/1‑138 (MQ=255)              ggCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAg                                                                                                                    >  1:109652/1‑132 (MQ=255)              ggCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAg                                                                                                                    <  2:109652/132‑1 (MQ=255)              ggCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGAt                                                                                                              >  1:232660/1‑138 (MQ=255)              ggCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGAt                                                                                                              >  2:348793/1‑138 (MQ=255)              ggCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGAt                                                                                                              >  1:94772/1‑138 (MQ=255)              ggCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGAt                                                                                                              >  1:637253/1‑138 (MQ=255)                                 gtgtGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGgcgcg                                                                                          >  1:284466/1‑139 (MQ=255)                                              tGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGgcgcg                                                                             <  2:623438/139‑1 (MQ=255)                                              tGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGgcgcg                                                                             <  2:585352/139‑1 (MQ=255)                                              tGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGgcgcg                                                                             <  1:348793/139‑1 (MQ=255)                                              tGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGgcgcg                                                                             <  2:345054/139‑1 (MQ=255)                                                     cacaTGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGttt                                                                       >  1:399110/1‑138 (MQ=255)                                                     cacaTGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGttt                                                                       >  2:254489/1‑138 (MQ=255)                                                        aTGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTttatt                                                                   <  2:284466/139‑1 (MQ=255)                                                        aTGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTttatt                                                                   <  1:590349/139‑1 (MQ=255)                                                        aTGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTttatt                                                                   <  1:601622/139‑1 (MQ=255)                                                           aCAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACg                                                                >  1:98927/1‑139 (MQ=255)                                                                  gAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCaga                                                         <  2:98927/139‑1 (MQ=255)                                                                              catgTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATTTTAGGGCGGGCCCTGTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGTGCGTGTTGGGCGCGCTGTTTTCTTACGCTCCAGAGAGTGCGGAAgt                                             <  2:259022/136‑2 (MQ=255)                                                                                 gTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCg                                           >  1:46416/1‑138 (MQ=255)                                                                                 gTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCg                                           >  2:159029/1‑138 (MQ=255)                                                                                       aaaTTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACctct                                    <  1:159029/139‑1 (MQ=255)                                                                                           tGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGtt                                >  1:426825/1‑139 (MQ=255)                                                                                            ggTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGgcg                                                                               <  1:36528/91‑1 (MQ=255)                                                                                            ggTGCGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGgcg                                                                               >  2:36528/1‑91 (MQ=255)                                                                                                cGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCgcgg                                                                                              <  2:29857/72‑1 (MQ=255)                                                                                                cGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCgcgg                                                                                              >  1:29857/1‑72 (MQ=255)                                                                                                cGGGGCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCgg                            >  1:437558/1‑138 (MQ=255)                                                                                                   ggCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCg                                           >  2:246351/1‑120 (MQ=255)                                                                                                   ggCAACCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCg                                           <  1:246351/120‑1 (MQ=255)                                                                                                       aCCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCgcggcg                                                                                            >  2:230723/1‑67 (MQ=255)                                                                                                       aCCCGCACATTTCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCgcggcg                                                                                            <  1:230723/67‑1 (MQ=255)                                                                                                                 ttCAGGATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCt          >  2:461946/1‑139 (MQ=255)                                                                                                                      gATGTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGa      >  2:279485/1‑138 (MQ=255)                                                                                                                         gTTAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACctct                                    >  1:95904/1‑105 (MQ=255)                                                                                                                         gTTAGGGATGACCCATTTTTCACAGCAAGATAACTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACctct                                    <  2:95904/105‑1 (MQ=255)                                                                                                                           tAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCagaga                                                       <  1:190976/84‑1 (MQ=255)                                                                                                                           tAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCagaga                                                       >  2:190976/1‑84 (MQ=255)                                                                                                                           tAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACt  >  1:217199/1‑137 (MQ=255)                                                                                                                           tAGGGATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACt  >  1:369205/1‑137 (MQ=255)                                                                                                                               |                                                                                                                                     AATCCTCATACTTGGCGGTGAGATGATCGGGCGTGTGCTCTTTTATGGCCTGCACATGACAGTAGGAATGGCTATTGCAGGTTAACAAATTGGTGCGGGGCAACCCGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACT  >  NZ_CP009273/1658636‑1658894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑