Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,678,263	C→T	R375R (CGC→CGT)	rstB →	two‑component system sensor histidine kinase RstB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,678,263	0	C	T	97.6%	162.9 / NA	42	R375R (CGC→CGT)	rstB	two‑component system sensor histidine kinase RstB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/20);  minor base G (0/1);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCT  >  NZ_CP009273/1678127‑1678387                                                                                                                                         |                                                                                                                             aCGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCt                                                                                                                            <  2:282543/139‑1 (MQ=255)         cGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATcc                                                                                                                      >  1:594199/1‑137 (MQ=255)         cGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATc                                                                                                                       >  2:346048/1‑136 (MQ=255)         cGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATc                                                                                                                       <  1:346048/136‑1 (MQ=255)               tGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCgg                                                                                                               >  2:352486/1‑138 (MQ=255)                            ttGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACcgg                                                                                                 <  2:167671/139‑1 (MQ=255)                                                   gggAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCaa                                                                          >  2:153003/1‑139 (MQ=255)                                                   gggAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCaa                                                                          <  1:614771/139‑1 (MQ=255)                                                    ggAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCt                                                                                                        <  2:375596/108‑1 (MQ=255)                                                    ggAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCt                                                                                                        >  1:375596/1‑108 (MQ=255)                                                       aTAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGt                                                                      >  2:162024/1‑139 (MQ=255)                                                        tAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTc                                                                     <  1:404885/139‑1 (MQ=255)                                                        tAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTc                                                                     <  1:428870/139‑1 (MQ=255)                                                        tAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTc                                                                     <  2:560302/139‑1 (MQ=255)                                                          gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   <  2:355333/139‑1 (MQ=255)                                                          gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   <  2:7691/139‑1 (MQ=255)                                                          gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   <  2:381770/139‑1 (MQ=255)                                                          gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   >  1:558075/1‑139 (MQ=255)                                                          gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   >  1:5579/1‑139 (MQ=255)                                                          gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   <  1:326775/139‑1 (MQ=255)                                                          gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   <  1:467984/139‑1 (MQ=255)                                                                          tgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATc                                                                                                                       <  2:257587/71‑1 (MQ=255)                                                                          tgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATc                                                                                                                       >  1:257587/1‑71 (MQ=255)                                                                          tgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAGCCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCa                                                         <  2:202422/133‑1 (MQ=255)                                                                          tgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAGCCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCa                                                         >  1:202422/1‑133 (MQ=255)                                                                          tgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGc                                                    >  2:480917/1‑138 (MQ=255)                                                                           gAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   >  1:393592/1‑122 (MQ=255)                                                                           gAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCa                                                                   <  2:393592/122‑1 (MQ=255)                                                                           gAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCaa                                                  >  1:455005/1‑139 (MQ=255)                                                                            aGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAAt                                                 >  1:323228/1‑139 (MQ=255)                                                                               atgatgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGACCCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATggg                                              <  2:569926/139‑1 (MQ=255)                                                                                   tgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGt                                                                      >  1:442414/1‑111 (MQ=255)                                                                                   tgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGt                                                                      <  2:442414/111‑1 (MQ=255)                                                                                         aGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCa                                                                           >  1:563648/1‑100 (MQ=255)                                                                                         aGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGGCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCa                                                                           <  2:563648/100‑1 (MQ=255)                                                                                                gCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATtgt                              >  1:403168/1‑138 (MQ=255)                                                                                                gCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATtgt                              >  1:99680/1‑138 (MQ=255)                                                                                                      gAAAACCGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGacac                         >  2:151075/1‑137 (MQ=255)                                                                                                           ccGCGAACATATCTTTGAACCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGaa                  >  1:230777/1‑139 (MQ=255)                                                                                                                            aaCCTTTTGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCt  <  2:230777/138‑1 (MQ=255)                                                                                                                                 tttGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCa                                                   <  2:418273/84‑1 (MQ=255)                                                                                                                                 tttGTTCGTCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCa                                                   >  1:418273/1‑84 (MQ=255)                                                                                                                                         |                                                                                                                             ACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCT  >  NZ_CP009273/1678127‑1678387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑