Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,695,442	T→G	F77V (TTT→GTT)	malY →	bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,695,442	0	T	G	100.0%	170.4 / NA	44	F77V (TTT→GTT)	malY	bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/29);  total (15/29)

CCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTG  >  NZ_CP009273/1695307‑1695555                                                                                                                                         |                                                                                                                  ccGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGgttt                                                                                                                <  1:329628/139‑1 (MQ=255)    ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCa                                                                                                             <  2:203167/139‑1 (MQ=255)    ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCa                                                                                                             >  2:519335/1‑139 (MQ=255)    ttCACGATTTCAGACATGGATTTGGCCACTGCCCCCTGCATTATCGAGGGGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCa                                                                                                             <  2:309592/139‑1 (MQ=255)     tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAc                                                                                                            >  1:622934/1‑139 (MQ=255)     tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAc                                                                                                            >  2:166345/1‑139 (MQ=255)     tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAc                                                                                                            >  2:607959/1‑139 (MQ=255)     tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAc                                                                                                            >  2:629192/1‑139 (MQ=255)       aCGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGGTTTCCAcc                                                                                                           <  1:9714/139‑1 (MQ=255)       aCGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAcc                                                                                                           <  2:120605/138‑1 (MQ=255)        cGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAcc                                                                                                           >  1:152374/1‑137 (MQ=255)                       aTTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCAt                                                                                          <  1:315146/139‑1 (MQ=255)                       aTTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCAt                                                                                          <  1:47591/139‑1 (MQ=255)                           tGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGa                                                                                       <  2:258970/138‑1 (MQ=255)                               aCTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATtctc                                                                                  <  2:377666/139‑1 (MQ=255)                                 tGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAcc                                                                                                           <  2:1824/112‑1 (MQ=255)                                 tGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCAcc                                                                                                           >  1:1824/1‑112 (MQ=255)                                   cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                               <  1:577542/138‑1 (MQ=255)                                   cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                               <  2:298026/138‑1 (MQ=255)                                   cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                               <  1:406429/138‑1 (MQ=255)                                     cccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACgg                                                                            <  1:321887/139‑1 (MQ=255)                                      ccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACggt                                                                           <  1:607959/139‑1 (MQ=255)                                            ttATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTa                                                                     <  1:436053/139‑1 (MQ=255)                                            ttATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTa                                                                     <  2:31339/139‑1 (MQ=255)                                              aTCGAGGCGCTGAATCAGCGCTTGATGCACGGCTTATTTGGCTACAGCCGCTGGAAACACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTAt                                                                    <  1:570820/138‑1 (MQ=255)                                               tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATtctc                                                                                  >  1:326350/1‑123 (MQ=255)                                               tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATtctc                                                                                  <  2:326350/123‑1 (MQ=255)                                               tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                               <  1:420180/126‑1 (MQ=255)                                               tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                               >  2:420180/1‑126 (MQ=255)                                                    gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCtt                                                             <  2:528063/139‑1 (MQ=255)                                                     cgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTACTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTc                                                            >  1:339529/1‑139 (MQ=255)                                                       cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                               <  1:469473/118‑1 (MQ=255)                                                       cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                               >  2:469473/1‑118 (MQ=255)                                                       cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTg                                                          >  1:92707/1‑139 (MQ=255)                                                           aTCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCAt                                                      <  1:331259/139‑1 (MQ=255)                                                                cgcCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCtata                                                 >  1:337073/1‑139 (MQ=255)                                                                 gcCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTatat                                                <  2:152374/139‑1 (MQ=255)                                                                      aTGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGttt                                           >  1:259114/1‑139 (MQ=255)                                                                                     ggCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGt                             <  2:622934/138‑1 (MQ=255)                                                                                       cTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAg                          <  1:630733/139‑1 (MQ=255)                                                                                          cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTgg                       >  1:312205/1‑139 (MQ=255)                                                                                                   gAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGaaa                <  2:311610/137‑1 (MQ=255)                                                                                                       aaCGATGAGTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTg          <  2:337073/139‑1 (MQ=255)                                                                                                               gTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCgtg  <  2:194262/139‑1 (MQ=255)                                                                                                               gTTTCTCGCGGCTATTGCCCACT‑GGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCgtg  <  2:92707/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                  CCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACT‑GGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTG  >  NZ_CP009273/1695307‑1695555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑