Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,747,881	A→C	G97G (GGT→GGG)	ydhV ←	aldehyde ferredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,747,881	0	A	C	100.0%	69.4 / NA	20	G97G (GGT→GGG)	ydhV	aldehyde ferredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/8);  total (12/8)

TAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTG  >  NZ_CP009273/1747754‑1748016                                                                                                                                |                                                                                                                                        taatGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGt                                                                                                                              >  2:268396/1‑139 (MQ=255) taatGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATACAGt                                                                                                                              >  2:433637/1‑139 (MQ=255)           gtATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCg                                                                                                                    <  1:63234/139‑1 (MQ=255)           gtATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCg                                                                                                                    >  2:137130/1‑139 (MQ=255)                 aGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACGACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCa                                                                                                              <  1:433637/139‑1 (MQ=255)                   cGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCa                                                                                                              >  1:464568/1‑137 (MQ=255)                                cgcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGc                                                                                                                      <  1:527725/116‑1 (MQ=255)                                cgcTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGc                                                                                                                      >  2:527725/1‑116 (MQ=255)                                     cAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCtt                                                                                           <  2:237111/138‑1 (MQ=255)                                               aCCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGt                                                                                >  1:119588/1‑139 (MQ=255)                                                cccATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGtt                                                                               <  1:365662/139‑1 (MQ=255)                                                          cATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTgg                                                                     <  1:359129/139‑1 (MQ=255)                                                          cATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTgg                                                                     >  2:627309/1‑139 (MQ=255)                                                           aTCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc                                                                    >  1:544117/1‑139 (MQ=255)                                                            tCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCg                                                                   >  1:242259/1‑139 (MQ=255)                                                                 gacTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACt                                                              <  2:513653/139‑1 (MQ=255)                                                                      aaTTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTc                                                         <  2:464568/139‑1 (MQ=255)                                                                                      aGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAg                                         >  2:479184/1‑139 (MQ=255)                                                                                                                 agaACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACtt              >  2:434192/1‑139 (MQ=255)                                                                                                                             ggCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTg  >  2:199509/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                        TAATGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTG  >  NZ_CP009273/1747754‑1748016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑