Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,120	A→G	I157V (ATT→GTT)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,120	0	A	G	100.0%	130.0 / NA	35	I157V (ATT→GTT)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

TGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAT  >  NZ_CP009273/1775988‑1776245                                                                                                                                     |                                                                                                                              tGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTgg                                                                                                                         <  1:505823/139‑1 (MQ=255)      gATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGAt                                                                                                                    >  2:100690/1‑139 (MQ=255)       aTGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGAt                                                                                                                    <  1:542730/138‑1 (MQ=255)       aTGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATg                                                                                                                   >  2:26804/1‑139 (MQ=255)                   gAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCg                                                                                                        <  2:4136/138‑1 (MQ=255)                     gCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATg                                                                                                     >  1:549442/1‑139 (MQ=255)                         gcgggcgCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTg                                                                                                 >  1:379877/1‑139 (MQ=255)                          cgggcgCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTgg                                                                                                >  2:407867/1‑139 (MQ=255)                             gcgcgcAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCcg                                                                                             <  2:72765/139‑1 (MQ=255)                                cgcAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCcgctc                                                                                          <  2:500046/139‑1 (MQ=255)                                               gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc                                                                           >  2:470406/1‑139 (MQ=255)                                                   tCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCt                                                                       >  1:538133/1‑139 (MQ=255)                                                          gACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATc                                                                <  1:470406/139‑1 (MQ=255)                                                            cgcgCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  1:375983/139‑1 (MQ=255)                                                                   gTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGcatca                                                         <  1:189773/137‑1 (MQ=255)                                                                             gAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGATTCCGATCCGCATCATTACGCTGTTgg                                             >  2:496443/1‑139 (MQ=255)                                                                                       cACTGGCGTGCAGGCTGGGGTTGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGtt                                         <  2:264922/133‑1 (MQ=255)                                                                                       cACTGGCGTGCAGGCTGGGGTTGATATTCTCGAAGCGAATGTGGGGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGtt                                         >  1:264922/1‑133 (MQ=255)                                                                                       cACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAagag                                   >  2:566578/1‑139 (MQ=255)                                                                                       cACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAagag                                   >  2:574296/1‑139 (MQ=255)                                                                                               tGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGtt                                                                                                                             <  1:572533/41‑1 (MQ=255)                                                                                               tGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGtt                                                                                                                             >  2:572533/1‑41 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  1:393163/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:549442/139‑1 (MQ=255)                                                                                                    gCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTca                      <  1:26804/139‑1 (MQ=255)                                                                                                            tgatATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa              <  2:379877/139‑1 (MQ=255)                                                                                                            tgatATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa              <  1:100690/139‑1 (MQ=255)                                                                                                              atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTaaa                                      <  1:488648/113‑1 (MQ=255)                                                                                                              atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTaaa                                      >  2:488648/1‑113 (MQ=255)                                                                                                              atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:465708/127‑1 (MQ=255)                                                                                                              atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        >  1:465708/1‑127 (MQ=255)                                                                                                                 ttCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTACCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGa          <  2:27569/138‑1 (MQ=255)                                                                                                                  tctcGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGtt                                               <  2:396722/100‑1 (MQ=255)                                                                                                                  tctcGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGtt                                               >  1:396722/1‑100 (MQ=255)                                                                                                                        aGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt  <  1:495558/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                              TGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAT  >  NZ_CP009273/1775988‑1776245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑