Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,806,054	T→C	S288S (AGT→AGC)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,806,054	0	T	C	100.0%	143.8 / NA	40	S288S (AGT→AGC)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/24);  total (16/24)

CCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAG  >  NZ_CP009273/1805927‑1806185                                                                                                                                |                                                                                                                                    ccAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                          <  1:566652/139‑1 (MQ=255)  cAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTAc                                                                                                                         <  1:24858/139‑1 (MQ=255)     aGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTc                                                                                                                      >  2:76031/1‑139 (MQ=255)         gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                   <  2:369455/138‑1 (MQ=255)         gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                   <  2:342665/138‑1 (MQ=255)         gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                   <  2:236257/138‑1 (MQ=255)         gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                   >  1:503973/1‑138 (MQ=255)             cAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGt                                                                                                              <  2:577142/139‑1 (MQ=255)                    tAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCt                                                                                                       >  1:321256/1‑139 (MQ=255)                                         aCTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTAcc                                                                                  <  1:76031/139‑1 (MQ=255)                                             ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGcc                                                                              >  2:145285/1‑139 (MQ=255)                                             ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                                               <  2:325521/138‑1 (MQ=255)                                             ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                                               <  1:449794/138‑1 (MQ=255)                                                  tttCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTc                                                                          >  1:491139/1‑138 (MQ=255)                                                       ttGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgc                                                                    <  2:16508/139‑1 (MQ=255)                                                         gTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                                   <  2:394104/138‑1 (MQ=255)                                                            gcgTCCTCCCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                                               <  2:403125/139‑1 (MQ=255)                                                            gcgTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                                               <  1:145285/139‑1 (MQ=255)                                                              gTCCTACCCCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTCCTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCt                                                             <  2:526601/139‑1 (MQ=255)                                                                         gTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTc                                                                          <  2:545834/115‑1 (MQ=255)                                                                         gTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTc                                                                          >  1:545834/1‑115 (MQ=255)                                                                         gTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGaa                                                  >  2:34422/1‑139 (MQ=255)                                                                          tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                                   <  1:16377/121‑1 (MQ=255)                                                                          tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                                   >  2:16377/1‑121 (MQ=255)                                                                            tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                   <  1:290075/71‑1 (MQ=255)                                                                            tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                   >  2:290075/1‑71 (MQ=255)                                                                              ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGc                                                                                                                                 >  2:306893/1‑55 (MQ=255)                                                                              ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGc                                                                                                                                 <  1:306893/55‑1 (MQ=255)                                                                              ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                                               <  2:480987/105‑1 (MQ=255)                                                                              ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                                               >  1:480987/1‑105 (MQ=255)                                                                                           cAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                         <  2:398662/66‑1 (MQ=255)                                                                                           cAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                                         >  1:398662/1‑66 (MQ=255)                                                                                           cAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcgcg                                >  2:212847/1‑139 (MQ=255)                                                                                           cAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcgcg                                <  2:503973/139‑1 (MQ=255)                                                                                                    gCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTggg                       <  1:34422/139‑1 (MQ=255)                                                                                                               ggCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTa                                                            >  2:443530/1‑91 (MQ=255)                                                                                                               ggCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTa                                                            <  1:443530/91‑1 (MQ=255)                                                                                                                      aTGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCg                     >  2:212087/1‑123 (MQ=255)                                                                                                                      aTGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCg                     <  1:212087/123‑1 (MQ=255)                                                                                                                         gCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAg  >  1:269871/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                    CCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAG  >  NZ_CP009273/1805927‑1806185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑