Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,809,486	A→C	T455P (ACT→CCT)	katE →	catalase HPII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,809,486	0	A	C	100.0%	93.1 / NA	25	T455P (ACT→CCT)	katE	catalase HPII
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/13);  total (12/13)

GTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTG  >  NZ_CP009273/1809359‑1809590                                                                                                                                |                                                                                                         gTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGc                                                                                                <  1:370666/138‑1 (MQ=255) gTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGc                                                                                                <  1:571983/138‑1 (MQ=255)         ataCCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTAcg                                                                                        >  2:482424/1‑138 (MQ=255)          taCCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTAcgaa                                                                                      <  2:166458/139‑1 (MQ=255)               aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTAcgaaccgaa                                                                                 >  2:589758/1‑139 (MQ=255)               aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTAcgaaccgaa                                                                                 >  1:287542/1‑139 (MQ=255)                 acacAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACt                                                                               >  2:500257/1‑139 (MQ=255)                                   ggtggGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGc                                                             >  1:476937/1‑139 (MQ=255)                                      gggCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACt                                                                               <  1:519527/118‑1 (MQ=255)                                      gggCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACt                                                                               >  2:519527/1‑118 (MQ=255)                                         ccGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCcgcgc                                                        >  1:135934/1‑138 (MQ=255)                                                    tGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACAccgccg                                            <  2:287542/139‑1 (MQ=255)                                                                  aCCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACgcgg                              <  2:438106/139‑1 (MQ=255)                                                                  aCCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACgcgg                              <  1:191274/139‑1 (MQ=255)                                                                               cccTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                   <  1:500257/137‑1 (MQ=255)                                                                               cccTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                   <  1:436821/137‑1 (MQ=255)                                                                               cccTTACCATAATTGCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                   <  2:203392/137‑1 (MQ=255)                                                                                    aCCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                   >  1:450475/1‑132 (MQ=255)                                                                                    aCCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                   <  2:450475/132‑1 (MQ=255)                                                                                         aaTTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                   <  1:393744/127‑1 (MQ=255)                                                                                         aaTTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                   >  2:393744/1‑127 (MQ=255)                                                                                         aaTTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAgc       >  1:100564/1‑139 (MQ=255)                                                                                             tCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGt   >  1:393268/1‑139 (MQ=255)                                                                                              ccAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTg  >  1:115446/1‑139 (MQ=255)                                                                                               cAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTg  <  1:541084/138‑1 (MQ=255)                                                                                                                                |                                                                                                         GTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTG  >  NZ_CP009273/1809359‑1809590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑