Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,819,028	T→C	E56G (GAA→GGA)	ves ←	environmental stress‑induced protein Ves

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,819,028	0	T	C	100.0%	66.5 / NA	18	E56G (GAA→GGA)	ves	environmental stress‑induced protein Ves
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

CCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCG  >  NZ_CP009273/1818900‑1819129                                                                                                                                 |                                                                                                      ccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCgg                                                                                              >  2:357958/1‑138 (MQ=255)           cGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGa                                                                                    >  2:280597/1‑138 (MQ=255)                      ccTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCAtt                                                                        <  2:277707/139‑1 (MQ=255)                      ccTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTACCCATGCCGGGAAACAAAGAAAACTCCCCAtt                                                                        <  1:555514/139‑1 (MQ=255)                                          aaaGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGc                                                    >  2:184011/1‑139 (MQ=255)                                          aaaGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGc                                                    >  1:10080/1‑139 (MQ=255)                                                  gAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGc                                            <  1:51095/139‑1 (MQ=255)                                                      ggTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGt                                        <  1:175538/139‑1 (MQ=255)                                                             aGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAAt                                  <  2:10080/138‑1 (MQ=255)                                                                          gCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGt                    <  1:315944/139‑1 (MQ=255)                                                                             gTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCga                                                                <  2:248776/92‑1 (MQ=255)                                                                             gTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCga                                                                >  1:248776/1‑92 (MQ=255)                                                                              tCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTggg                 >  2:226657/1‑138 (MQ=255)                                                                              tCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTggg                 >  1:175766/1‑138 (MQ=255)                                                                                          aGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAAttt    >  1:524654/1‑139 (MQ=255)                                                                                            gAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTcg  <  2:453954/139‑1 (MQ=255)                                                                                                 tctcGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggatg                                                          >  1:608594/1‑78 (MQ=255)                                                                                                 tctcGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggatg                                                          <  2:608594/78‑1 (MQ=255)                                                                                                                                 |                                                                                                      CCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCG  >  NZ_CP009273/1818900‑1819129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑