Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,865,194	A→C	N185H (AAC→CAC)	cdgI →	GGDEF domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,865,194	0	A	C	100.0%	110.0 / NA	29	N185H (AAC→CAC)	cdgI	GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/10);  total (19/10)

TAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTC  >  NZ_CP009273/1865060‑1865329                                                                                                                                       |                                                                                                                                        tAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGaccaca                                                                                                                                      <  2:480323/138‑1 (MQ=255)           tAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGac                                                                                                                              <  1:291374/136‑1 (MQ=255)           tAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGac                                                                                                                              >  2:291374/1‑136 (MQ=255)                                   gATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTg                                                                                                   >  1:281566/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  2:70806/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  2:488009/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  2:378695/1‑139 (MQ=255)                                    aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                                  >  2:300988/1‑139 (MQ=255)                                     tCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGtt                                                                                                 >  2:120823/1‑139 (MQ=255)                                       cAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGc                                                                                               >  1:73840/1‑139 (MQ=255)                                         gACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTg                                                                                             >  2:274694/1‑139 (MQ=255)                                          aCAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTgg                                                                                            >  2:609971/1‑139 (MQ=255)                                              aGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGa                                                                                                                               >  1:243859/1‑100 (MQ=255)                                              aGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGa                                                                                                                               <  2:243859/100‑1 (MQ=255)                                               gTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCt                                                                                        >  2:614849/1‑138 (MQ=255)                                                                       tatTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcccc                                                                 <  2:281566/137‑1 (MQ=255)                                                                                       aGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTTGCACACAATCTg                                                <  2:23622/138‑1 (MQ=255)                                                                                         ttttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                             <  1:609971/139‑1 (MQ=255)                                                                                              tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTta                                        >  2:277223/1‑139 (MQ=255)                                                                                                  tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTCTAGTTCTTCCCCTTCTGGCACCCAATCTGAGCAGTTATAAc                                    <  2:613911/139‑1 (MQ=255)                                                                                                   ttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACg                                   >  2:166510/1‑139 (MQ=255)                                                                                                       ccTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACGACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGa                               <  1:389390/139‑1 (MQ=255)                                                                                                       ccTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGa                               <  1:300988/139‑1 (MQ=255)                                                                                                                  ctctTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                             >  2:287869/1‑114 (MQ=255)                                                                                                                  ctctTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                             <  1:287869/114‑1 (MQ=255)                                                                                                                               gAAAAGTCCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa       <  1:353331/139‑1 (MQ=255)                                                                                                                               gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa       >  2:528073/1‑139 (MQ=255)                                                                                                                               gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa       >  1:554821/1‑139 (MQ=255)                                                                                                                               gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa       >  1:630840/1‑139 (MQ=255)                                                                                                                                    gACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTc  >  1:630551/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        TAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTC  >  NZ_CP009273/1865060‑1865329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑