Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	51,400	G→C	G69G (GGC→GGG)	apaG ←	Co2+/Mg2+ efflux protein ApaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	51,400	0	G	C	100.0%	118.4 / NA	32	G69G (GGC→GGG)	apaG	Co2+/Mg2+ efflux protein ApaG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/14);  total (18/14)

TCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCA  >  NZ_CP009273/51296‑51535                                                                                                         |                                                                                                                                        tCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCa                                                                                                        <  1:575739/138‑1 (MQ=255)   aTCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACg                                                                                                      <  2:350399/138‑1 (MQ=255)        tCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgccatt                                                                                                >  2:264919/1‑139 (MQ=255)                     aCCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGTTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGAt                                                                                    >  2:26356/1‑138 (MQ=255)                     aCCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCg                                                                                                                          <  1:579089/100‑1 (MQ=255)                     aCCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCg                                                                                                                          >  2:579089/1‑100 (MQ=255)                          gCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGc                                                                                >  1:196304/1‑137 (MQ=255)                                tGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACg                                                                        >  2:463294/1‑139 (MQ=255)                                   ccAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGccc                                                                     >  2:129196/1‑139 (MQ=255)                                     aGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAAc                                                                         >  2:477355/1‑133 (MQ=255)                                     aGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAAc                                                                         <  1:477355/133‑1 (MQ=255)                                          ggTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGAt                                                                                    >  1:184523/1‑117 (MQ=255)                                          ggTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGAt                                                                                    <  2:184523/117‑1 (MQ=255)                                                     ttGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGgcgc                                                   <  1:26356/139‑1 (MQ=255)                                                          ccGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGcc                                              >  2:575172/1‑139 (MQ=255)                                                             cTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgcc                                                                                                   >  1:100345/1‑83 (MQ=255)                                                             cTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGgcc                                                                                                   <  2:100345/83‑1 (MQ=255)                                                                gtgtACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGAt                                        >  1:630824/1‑139 (MQ=255)                                                                     cTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCg                                    <  1:129196/138‑1 (MQ=255)                                                                        gTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCt                                                                                                                             <  1:434172/46‑1 (MQ=255)                                                                        gTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCt                                                                                                                             >  2:434172/1‑46 (MQ=255)                                                                        gTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATg                                >  2:149962/1‑139 (MQ=255)                                                                        gTACTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATg                                >  1:495333/1‑139 (MQ=255)                                                                          aCTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGa                                         >  1:141954/1‑128 (MQ=255)                                                                          aCTCTTCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGa                                         <  2:141954/128‑1 (MQ=255)                                                                              ttCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTa                            <  1:463294/137‑1 (MQ=255)                                                                              ttCGCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTa                            <  2:346568/137‑1 (MQ=255)                                                                                 gCCAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTACGGt                        <  1:264919/138‑1 (MQ=255)                                                                                  ccAGGCGCGATAAGTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTACGGta                       >  1:201877/1‑138 (MQ=255)                                                                                               gTGGCTGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAaacgta         <  1:413155/139‑1 (MQ=255)                                                                                                    tGGACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGtt    >  1:198479/1‑139 (MQ=255)                                                                                                      gACCCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCa  >  1:542576/1‑139 (MQ=255)                                                                                                         |                                                                                                                                        TCATCGATCATTTCGTAGTGACCCTGCATGGTGCCCAGCGGGGTTTCAATGATTGCACCGCTGGTGTACTGGTACTCTTCGCCAGGCGCGATAAGTGGCTGGACGCCAACCACTCCTTCGCCCTGGACTTCGGTTTCACGGCCATTGCCATTGGTGATCAGCCAGTAACGCCCCAACAACTGCACTGGCGCTCGCCCCAGATTGCGTATGGTTACGGTATAAGCAAAAACGTAACGTTCA  >  NZ_CP009273/51296‑51535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑