Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,919,822	T→C	F42F (TTT→TTC)	yobB →	carbon‑nitrogen hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,919,822	0	T	C	100.0%	152.9 / NA	39	F42F (TTT→TTC)	yobB	carbon‑nitrogen hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

GGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTT  >  NZ_CP009273/1919687‑1919954                                                                                                                                        |                                                                                                                                     ggAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCa                                                                                                                                   <  1:16788/139‑1 (MQ=255) ggAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCa                                                                                                                                   <  2:625244/139‑1 (MQ=255)   aaGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATc                                                                                                                                 <  2:356263/139‑1 (MQ=255)      tCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGct                                                                                                                              <  2:117595/139‑1 (MQ=255)              tCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTa                                                                                                                       <  2:58433/138‑1 (MQ=255)                           ttGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGatta                                                                                                         >  1:282241/1‑139 (MQ=255)                                    cgcAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACg                                                                                                >  2:438919/1‑139 (MQ=255)                                     gcAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGt                                                                                               >  1:14149/1‑139 (MQ=255)                                      cAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg                                                                                              <  1:550140/139‑1 (MQ=255)                                           tgcgCCTCGTAAATCCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCTCTTTCTTTACTGGGGGGTGATTATTCGCGACGTGCCCtt                                                                                         <  2:508203/136‑1 (MQ=255)                                              gCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg                                                                                              >  2:466884/1‑131 (MQ=255)                                              gCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTg                                                                                              <  1:466884/131‑1 (MQ=255)                                                   gTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc                                                                                  >  2:636061/1‑138 (MQ=255)                                                   gTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc                                                                                  >  2:324312/1‑138 (MQ=255)                                                   gTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGccc                                                                                  >  1:23988/1‑138 (MQ=255)                                                    tAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCa                                                                                >  1:532622/1‑139 (MQ=255)                                                      aaaCCGCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCAcc                                                                              <  2:304275/139‑1 (MQ=255)                                                              ctcacCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctct                                                                      <  2:561942/139‑1 (MQ=255)                                                                 acCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctctc                                                                     <  1:324312/137‑1 (MQ=255)                                                                 acCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCa                                                                    <  1:636061/138‑1 (MQ=255)                                                                     aaCAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGat                                                                                                           >  1:377485/1‑95 (MQ=255)                                                                     aaCAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGat                                                                                                           <  2:377485/95‑1 (MQ=255)                                                                           tAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGAccc                                                         >  1:594366/1‑139 (MQ=255)                                                                             gCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGc                                                       >  2:209621/1‑139 (MQ=255)                                                                                catcatCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTc                                                                                                      >  1:419854/1‑89 (MQ=255)                                                                                catcatCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTc                                                                                                      <  2:419854/89‑1 (MQ=255)                                                                                    atcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTa                                                >  2:261991/1‑139 (MQ=255)                                                                                         gAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctct                                                                      >  2:360774/1‑112 (MQ=255)                                                                                         gAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGAtctct                                                                      <  1:360774/112‑1 (MQ=255)                                                                                                    ggCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTgg                                >  1:347268/1‑139 (MQ=255)                                                                                                      ccgccgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCa                                                                    >  1:46525/1‑101 (MQ=255)                                                                                                      ccgccgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCa                                                                    <  2:46525/101‑1 (MQ=255)                                                                                                      ccgccgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGc                               >  2:111460/1‑138 (MQ=255)                                                                                                       cgccgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGa                             <  1:91054/139‑1 (MQ=255)                                                                                                          cgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCAcc                                                                              >  2:580263/1‑87 (MQ=255)                                                                                                          cgccAGGCAACAATGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCAcc                                                                              <  1:580263/87‑1 (MQ=255)                                                                                                                       tGTCAGCTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCg             >  1:488028/1‑139 (MQ=255)                                                                                                                             cTGTTAGTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTc       <  2:347268/139‑1 (MQ=255)                                                                                                                                   gTTTTCCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGtt  <  1:261991/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GGAAGTCATTATGTCGTTCTGGAAAGTTGCAGCAGCGCAATATGAGCCTCGTAAAACCTCACTCACCGAACAGGTAGCTCATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTT  >  NZ_CP009273/1919687‑1919954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑