Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,948,189	T→G	V164G (GTG→GGG)	cmoB →	tRNA 5‑methoxyuridine(34)/uridine 5‑oxyacetic acid(34) synthase CmoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,948,189	0	T	G	100.0%	150.8 / NA	43	V164G (GTG→GGG)	cmoB	tRNA 5‑methoxyuridine(34)/uridine 5‑oxyacetic acid(34) synthase CmoB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/21);  total (22/21)

ATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGAGC  >  NZ_CP009273/1948057‑1948320                                                                                                                                     |                                                                                                                                    aTTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTa                                                                                                                                <  1:493495/138‑1 (MQ=255)   ttAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAac                                                                                                                             >  1:307093/1‑139 (MQ=255)      aCCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTa                                                                                                                                <  2:517604/133‑1 (MQ=255)      aCCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTa                                                                                                                                >  1:517604/1‑133 (MQ=255)       ccGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         <  2:182478/139‑1 (MQ=255)       ccGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         <  2:428746/139‑1 (MQ=255)       ccGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         <  2:307093/139‑1 (MQ=255)           aCGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTggg                                                                                                                      >  2:253338/1‑138 (MQ=255)           aCGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGt                                                                                                                     <  2:28546/139‑1 (MQ=255)                             cGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCAt                                                                                                   >  1:336715/1‑139 (MQ=255)                              ggCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCAtt                                                                                                  >  1:204232/1‑139 (MQ=255)                                  gCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                               >  2:421434/1‑138 (MQ=255)                                   cGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                               <  2:143739/137‑1 (MQ=255)                                        gCGGTTATCACAGGTGGCTCATGATTGGCTCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAATCTACTGGGTAATTATCAGCGCGCGCATTTGTTACCGtt                                                                                        <  2:528335/139‑1 (MQ=255)                                           gTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAgg                                                                                     >  1:322125/1‑139 (MQ=255)                                                     gtgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         <  1:390950/93‑1 (MQ=255)                                                     gtgGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         >  2:390950/1‑93 (MQ=255)                                                         cgcATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAACCTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTc                                                                       >  1:273542/1‑139 (MQ=255)                                                           cATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCg                                                                     >  1:171818/1‑139 (MQ=255)                                                                    cgcAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGaaa                                                            <  1:32838/139‑1 (MQ=255)                                                                        ggCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCt                                                        >  1:544201/1‑139 (MQ=255)                                                                         gcgcgcACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         >  2:449626/1‑73 (MQ=255)                                                                         gcgcgcACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         <  1:449626/73‑1 (MQ=255)                                                                              cACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                               >  2:504395/1‑94 (MQ=255)                                                                              cACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                               <  1:504395/94‑1 (MQ=255)                                                                                   cgcgGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTc                                             >  1:354298/1‑139 (MQ=255)                                                                                    gcgGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTc                                             <  2:204232/138‑1 (MQ=255)                                                                                           ggATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTa                                                                                             >  1:6493/1‑83 (MQ=255)                                                                                           ggATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTa                                                                                             <  2:6493/83‑1 (MQ=255)                                                                                                    cTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTGGATGGTAGTGCt                            >  2:185933/1‑139 (MQ=255)                                                                                                     tACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         >  2:105746/1‑45 (MQ=255)                                                                                                     tACGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAactact                                                                                                                         <  1:105746/45‑1 (MQ=255)                                                                                                       cGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                               <  2:179777/69‑1 (MQ=255)                                                                                                       cGCAACTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGt                                                                                               >  1:179777/1‑69 (MQ=255)                                                                                                            cTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCAt                                                                                                   <  1:479467/60‑1 (MQ=255)                                                                                                            cTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCAt                                                                                                   >  2:479467/1‑60 (MQ=255)                                                                                                            cTGTTTCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATc                    >  2:276966/1‑139 (MQ=255)                                                                                                               tttCTGTGCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGcc                 >  2:395650/1‑139 (MQ=255)                                                                                                                     tgCCAGTTTGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGtt              <  2:354298/136‑1 (MQ=255)                                                                                                                           tttGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTg      >  2:106074/1‑138 (MQ=255)                                                                                                                             tGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGa    <  1:253338/138‑1 (MQ=255)                                                                                                                             tGAAGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGa    <  2:544201/138‑1 (MQ=255)                                                                                                                               aaGCGGGGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGAGc  <  2:359879/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    ATTTAACCGGACGCACCATTCTGGATGTCGGCTGCGGCAGCGGTTATCACATGTGGCGCATGATTGGCGCAGGCGCGCACCTCGCGGTGGGGATCGACCCTACGCAACTGTTTCTGTGCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTCGATGGGCGTGCTTTACCATCGCCGTTCACCGCTGGAGC  >  NZ_CP009273/1948057‑1948320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑