Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,968,121	A→G	G487G (GGT→GGC)	cheA ←	chemotaxis protein CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,968,121	0	A	G	100.0%	95.0 / NA	26	G487G (GGT→GGC)	cheA	chemotaxis protein CheA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/9);  total (17/9)

ATAACAGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTC  >  NZ_CP009273/1967987‑1968237                                                                                                                                       |                                                                                                                     aTAACAGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGc                                                                                                                  >  1:478163/1‑139 (MQ=255)  tAACAGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGgccgcc                                                                                                                 >  2:588510/1‑139 (MQ=255)    aCAGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGccc                                                                                                                <  1:118823/138‑1 (MQ=255)    aCAGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGccc                                                                                                                <  2:478163/138‑1 (MQ=255)    aCAGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGccc                                                                                                                <  2:142034/138‑1 (MQ=255)      aGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGccc                                                                                                                <  1:230060/136‑1 (MQ=255)      aGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGccc                                                                                                                >  2:230060/1‑136 (MQ=255)      aGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCAtc                                                                                                             >  1:316831/1‑139 (MQ=255)        cATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCAtctt                                                                                                           >  1:172497/1‑139 (MQ=255)        cATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCAtctt                                                                                                           >  1:620860/1‑139 (MQ=255)        cATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCAtctt                                                                                                           >  1:631984/1‑139 (MQ=255)          ttCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCAtcttct                                                                                                         >  2:413839/1‑139 (MQ=255)                                       aaCGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCg                                                                            <  2:620860/139‑1 (MQ=255)                                        aCGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCgc                                                                           >  2:264346/1‑138 (MQ=255)                                                            gAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCg                                                       >  2:11025/1‑139 (MQ=255)                                                                 tGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGAc                                                  <  2:124392/139‑1 (MQ=255)                                                                      aGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGct                                             <  1:26111/139‑1 (MQ=255)                                                                       gCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGctc                                            >  1:422215/1‑139 (MQ=255)                                                                                  aGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGt                                                     >  2:174806/1‑119 (MQ=255)                                                                                  aGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGt                                                     <  1:174806/119‑1 (MQ=255)                                                                                                          gTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCCGCATcg         >  2:288446/1‑139 (MQ=255)                                                                                                          gTACCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATcg         >  2:289598/1‑139 (MQ=255)                                                                                                            aCCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAg                           >  1:469024/1‑119 (MQ=255)                                                                                                            aCCCTGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAg                           <  2:469024/119‑1 (MQ=255)                                                                                                                tGCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACtt   >  2:573521/1‑139 (MQ=255)                                                                                                                tGCTTCGACTGGATTTCGACATGGCCGCCCACCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACtt   >  1:63847/1‑139 (MQ=255)                                                                                                                 gCTTCGACTGGATTTCGACATGGCCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTtc  >  1:213182/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                     ATAACAGCATTCAGCGGCAGAATGAAAACTTCATCCGCAACGCGTACGGACATGCCGTCGAGGATGGCCAGCGTCAGCGGCAGTAAAATGCGGATCGTAGTGCCAGTACCCTGCTTCGACTGGATTTCGACATGACCGCCCATCTTCTGGATATTACGTTTAACGACGTCCATGCCGACGCCGCGCCCGGAGACGTCGGTGACCTGCTCTGCCGTGGAGAAGCCAGGTGCAAATATCAGCATCGCGACTTC  >  NZ_CP009273/1967987‑1968237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑