Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,980,983	T→G	intergenic (+74/+5)	ftnB → / ← yecJ	non‑heme ferritin‑like protein/DUF2766 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,980,983	0	T	G	100.0%	111.3 / NA	32	intergenic (+74/+5)	ftnB/yecJ	non‑heme ferritin‑like protein/DUF2766 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/16);  total (16/16)

TGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATA  >  NZ_CP009273/1980853‑1981099                                                                                                                                   |                                                                                                                     tgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTaa                                                                                                              <  1:411909/139‑1 (MQ=255) tgCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTa                                                                                                               <  2:321722/138‑1 (MQ=255)   cccTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAAt                                                                                                             <  2:389989/138‑1 (MQ=255)        tgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGAtt                                                                                                       >  2:456138/1‑139 (MQ=255)             accgaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCAccc                                                                                                  >  2:593447/1‑139 (MQ=255)                gaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCtgt                                                                                               >  2:459393/1‑139 (MQ=255)                  ccAAGATGTTCTGAATGTCGTGTCACACCAGCTGAATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGCAGCAGCGGGTTTTTTAATGGGATTAACCCTGTgg                                                                                             >  2:456200/1‑139 (MQ=255)                  ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTgg                                                                                             >  1:323364/1‑139 (MQ=255)                  ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTgg                                                                                             >  2:510840/1‑139 (MQ=255)                  ccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTgg                                                                                             >  1:67667/1‑139 (MQ=255)                      cATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTa                                                                                         <  2:130475/139‑1 (MQ=255)                          ttCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACt                                                                                     <  1:510840/139‑1 (MQ=255)                               aaTGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAg                                                                                 <  1:501615/138‑1 (MQ=255)                                           cacCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGcgcg                                                                    <  2:315796/139‑1 (MQ=255)                                              cAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATg                                                                 >  2:329916/1‑139 (MQ=255)                                                    cATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCtttt                                                           >  1:130722/1‑139 (MQ=255)                                                         atcatcatcGGCGCTAATGCATTGCTCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATTGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGAt                                                      <  1:456200/139‑1 (MQ=255)                                                                  ggCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCa                                              <  1:28092/138‑1 (MQ=255)                                                                         aTGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGAcc                                      <  1:456138/139‑1 (MQ=255)                                                                                 cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCtg                                                                                                <  2:99051/73‑1 (MQ=255)                                                                                 cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCtg                                                                                                >  1:99051/1‑73 (MQ=255)                                                                                 cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgc                              >  1:461976/1‑139 (MQ=255)                                                                                   ccGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcgg                            >  2:281321/1‑139 (MQ=255)                                                                                   ccGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcgg                            >  2:6960/1‑139 (MQ=255)                                                                                     gATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcggcg                          <  1:6960/139‑1 (MQ=255)                                                                                      aTGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGcc                                                  >  1:615461/1‑114 (MQ=255)                                                                                      aTGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGcc                                                  <  2:615461/114‑1 (MQ=255)                                                                                      aTGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTgcggcgg                         >  2:66221/1‑139 (MQ=255)                                                                                           ggTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGa                    >  2:624831/1‑139 (MQ=255)                                                                                                   gaAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGt            <  2:134813/139‑1 (MQ=255)                                                                                                   gaAACCGCTGCCTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGt            <  2:461976/139‑1 (MQ=255)                                                                                                             ccTCATCTGTTTGAAGCAGCGGGTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGata  <  1:593447/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                     TGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATA  >  NZ_CP009273/1980853‑1981099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑