Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,982,130	G→A	intergenic (+104/‑67)	yecR → / → ftnA	YecR‑like lipofamily protein/non‑heme ferritin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,982,130	0	G	A	100.0%	48.0 / NA	13	intergenic (+104/‑67)	yecR/ftnA	YecR‑like lipofamily protein/non‑heme ferritin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/10);  total (3/10)

GCCATCATTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTA  >  NZ_CP009273/1981995‑1982252                                                                                                                                        |                                                                                                                           gCCATCATTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACa                                                                                                                          >  1:83481/1‑138 (MQ=255)    atcatTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAaat                                                                                                                       >  2:285240/1‑138 (MQ=255)            gCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATaataaaata                                                                                                              <  2:444424/139‑1 (MQ=255)                                      ttAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGcaa                                                                                     <  2:156112/138‑1 (MQ=255)                                      ttAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGcaa                                                                                     <  2:83481/138‑1 (MQ=255)                                      ttAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCAACAGcaa                                                                                     >  2:171280/1‑138 (MQ=255)                                            aGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAatat                                                                              <  2:581294/139‑1 (MQ=255)                                                          cGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTtgtg                                                                    <  2:461979/135‑1 (MQ=255)                                                          cGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAg                                                                 <  1:285240/138‑1 (MQ=255)                                                                                                          ttAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGAt                 <  1:220059/138‑1 (MQ=255)                                                                                                          ttAATAATTGAAGTTTATATTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGAt                 <  1:37577/138‑1 (MQ=255)                                                                                                                         tataTTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTa  <  2:358602/138‑1 (MQ=255)                                                                                                                         tataTTTTACCTTTTACAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTa  <  2:60918/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                           GCCATCATTCCGCCTTCCCTGTCTTTCTCTAAATATTTTAATCAGCGAGGGGATCTTCGCTGATTAAAGAAATAGCGGTAATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAATGATTGAAAAACTTAATGAGCAGATGAACCTGGAACTGTA  >  NZ_CP009273/1981995‑1982252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑