Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,069,352	A→C	T365P (ACA→CCA)	BW25113_RS10450 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,069,352	0	A	C	100.0%	74.5 / NA	20	T365P (ACA→CCA)	BW25113_RS10450	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/11);  total (9/11)

GTGGATGAGCTTCGTATCTGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATGAGAAAATGTTTGCTTCCGGAGGG  >  NZ_CP009273/2069238‑2069480                                                                                                                   |                                                                                                                                 gTGGATGAGCTTCGTATCTGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAAc                                                                                                          <  1:439978/139‑1 (MQ=255) gTGGATGAGCTTCGTATCTGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAAc                                                                                                          <  2:176311/139‑1 (MQ=255) gTGGATGAGCTTCGTATCTGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAAc                                                                                                          <  2:490979/139‑1 (MQ=255) gTGGATGAGCTTCGTATCTGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAAc                                                                                                          <  2:417927/139‑1 (MQ=255)                     tgCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCttt                                                                                         <  2:233413/136‑1 (MQ=255)                                                   tGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATtct                                                         >  1:31464/1‑138 (MQ=255)                                                               tCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCg                                            <  2:389059/139‑1 (MQ=255)                                                                                    gTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGc                                                                                                        <  1:220455/58‑1 (MQ=255)                                                                                    gTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGc                                                                                                        >  2:220455/1‑58 (MQ=255)                                                                                    gTACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAAt                          >  2:450811/1‑136 (MQ=255)                                                                                     tACTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATgag                       >  1:416134/1‑138 (MQ=255)                                                                                       cTGCTCCTCTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATGAGaa                     <  2:31464/138‑1 (MQ=255)                                                                                          ctcctcTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAAcc                                      >  1:620595/1‑118 (MQ=255)                                                                                          ctcctcTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAAcc                                      <  2:620595/118‑1 (MQ=255)                                                                                          ctcctcTCTTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATGAGAAAATg                 >  1:611077/1‑139 (MQ=255)                                                                                               ctctTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATgaga                      >  1:247802/1‑129 (MQ=255)                                                                                               ctctTTGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATgaga                      <  2:247802/129‑1 (MQ=255)                                                                                                    tGTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATGAGAAAATGTTTGCTTCCg       >  2:18805/1‑139 (MQ=255)                                                                                                     gTCGCGAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATGAGAAAATGTTTGCTTCCg       <  2:611077/138‑1 (MQ=255)                                                                                                         cgAAACAGAACCACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATGAGAAAATGTTTGCTTCCGGAggg  >  2:69059/1‑139 (MQ=255)                                                                                                                   |                                                                                                                                 GTGGATGAGCTTCGTATCTGTGCCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTACAACAGCTTTATTTCCGATGCAACGGCGAATGGTCTGATTCTCATGAAACTGCCGGAAACCTGGAGTACAAATGAGAAAATGTTTGCTTCCGGAGGG  >  NZ_CP009273/2069238‑2069480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑