Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,141,824	T→C	S224P (TCA→CCA)	yegD →	molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,141,824	0	T	C	100.0%	77.5 / NA	23	S224P (TCA→CCA)	yegD	molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/16);  total (7/16)

CGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCACTGC  >  NZ_CP009273/2141688‑2141956                                                                                                                                         |                                                                                                                                     cgcgGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCa                                                                                                                                    <  2:51947/139‑1 (MQ=255)       gAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATtgctg                                                                                                                              <  2:381581/139‑1 (MQ=255)                            cGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGACTGACTGTCCCTTGCTGCTGATGGGGGCGCAGGGGCGt                                                                                                         >  1:180804/1‑139 (MQ=255)                              tGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTc                                                                                                       <  1:44059/139‑1 (MQ=255)                                gTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTcg                                                                                                      <  1:501039/138‑1 (MQ=255)                                           cGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGGATGACTGTCCCTTGCTGCTGGTGGGGGCGCGGGGGGGGTCGCGTCCCGGTCGt                                                                                          >  2:243730/1‑139 (MQ=255)                                                  gTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAagcca                                                                                   <  2:378362/139‑1 (MQ=255)                                                     gcgGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAagccagcc                                                                                <  1:328849/139‑1 (MQ=255)                                                       ggctgggctgGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctg                                                                              <  2:71265/139‑1 (MQ=255)                                                         ctgggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctgct                                                                            <  2:74926/139‑1 (MQ=255)                                                            ggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctgctg                                                                           <  2:289152/137‑1 (MQ=255)                                                             gctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGGGGGGAGGCGGACAGTCCCTTGGTGGTGGTGGGGGCGCCGGGGGGGTGGCGTCCCGGTTGTGCAGCCAGCCCGCTgggg                                                                        >  2:10343/1‑138 (MQ=255)                                                                 gATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTca                                                                      <  2:554855/137‑1 (MQ=255)                                                                      cGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGGCTGAATGACCATTGCTGCTGGTGGGGGCGGCGGGGGGGTCGGGGCTCGATCGTGGAGGCAGCCCGCTGGGGCCCCGTggg                                                               >  2:248623/1‑138 (MQ=255)                                                                         aGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGc                                                            <  1:528467/139‑1 (MQ=255)                                                                           ccACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGGCTGGCTGTCCCTTGCTGGTGGTGGGGGCGGCGGGGGGGTCGCGTCCCGGTCGGGAAGGCCGCCTGCTGGGGCACAGTGGGTGGCg                                                          >  2:434920/1‑139 (MQ=255)                                                                                             aaCGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCt                                        <  1:413313/139‑1 (MQ=255)                                                                                                  gTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGata                                   <  2:10279/139‑1 (MQ=255)                                                                                                                           gtACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGa           <  1:639126/138‑1 (MQ=255)                                                                                                                              cGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGc        <  1:463832/138‑1 (MQ=255)                                                                                                                                actgactgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGGAAAGATCTGGGGATTGCGCTGGGGGTTTAAAACCTGATggccc     >  2:463832/1‑135 (MQ=255)                                                                                                                                   gactgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActgc  >  1:462228/1‑139 (MQ=255)                                                                                                                                    actgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActgc  <  1:10343/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCACTGC  >  NZ_CP009273/2141688‑2141956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑