Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,154,426	A→G	I854V (ATC→GTC)	mdtC →	multidrug efflux RND transporter permease subunit MdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,154,426	0	A	G	100.0%	118.0 / NA	33	I854V (ATC→GTC)	mdtC	multidrug efflux RND transporter permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/19);  total (14/19)

CCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTCTCTCCACCCTGCCCTCGGCGGGCGTTGGAGCGCTGTTGGCGCTGGAGCTGTTCA  >  NZ_CP009273/2154291‑2154561                                                                                                                                        |                                                                                                                                        ccGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTcc                                                                                                                                      >  2:8660/1‑139 (MQ=255)                     ggACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGcc                                                                                                                  <  1:528326/139‑1 (MQ=255)                     ggACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACACGCAGGTGGTCCTGATTATCGCCGCCATCGcc                                                                                                                  <  1:561605/139‑1 (MQ=255)                      gACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCa                                                                                                                 <  2:80493/139‑1 (MQ=255)                      gACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCa                                                                                                                 <  1:584581/139‑1 (MQ=255)                      gACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCa                                                                                                                 <  1:8660/139‑1 (MQ=255)                       aCGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCAc                                                                                                                <  2:460797/139‑1 (MQ=255)                                   cgatcgatCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGcc                                                                                                                  <  1:364503/125‑1 (MQ=255)                                   cgatcgatCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGcc                                                                                                                  >  2:364503/1‑125 (MQ=255)                                         atcgCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTggg                                                                                              <  2:314557/139‑1 (MQ=255)                                                  tGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTc                                                                                                                                       <  1:289112/89‑1 (MQ=255)                                                  tGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTc                                                                                                                                       >  2:289112/1‑89 (MQ=255)                                                             ggTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTa                                                                            <  2:618994/137‑1 (MQ=255)                                                               tgtgCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTg                                                                                                   >  1:190464/1‑112 (MQ=255)                                                               tgtgCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTg                                                                                                   <  2:190464/112‑1 (MQ=255)                                                                            gggctcgGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTAGCGCCGCCAGCGCCACGGTGTATATCGTGCTGGGTATCCGTTACGAGAGTTACGTACATCCGCTGACGa                                                           <  2:87616/134‑1 (MQ=255)                                                                               cgcgGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATtc                                                        >  2:367464/1‑139 (MQ=255)                                                                                  ggCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGAt                                                          <  1:59222/134‑1 (MQ=255)                                                                                  ggCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGAt                                                          >  2:59222/1‑134 (MQ=255)                                                                                  ggCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATtctct                                                     <  1:367464/139‑1 (MQ=255)                                                                                       ttttGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCAc                                                                                                                >  1:455303/1‑75 (MQ=255)                                                                                       ttttGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCAc                                                                                                                <  2:455303/75‑1 (MQ=255)                                                                                                               ccAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGtata                                                                                                        >  2:304927/1‑59 (MQ=255)                                                                                                               ccAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGtata                                                                                                        <  1:304927/59‑1 (MQ=255)                                                                                                               ccAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTCTCTCCACCCTGCCCTCGGCGGGCGTTGGAgcg                        >  1:473117/1‑139 (MQ=255)                                                                                                               ccAGGAGACGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTCTCTCCACCCTGCCCTCGGCGGGCGTTGGAgcg                        >  2:336228/1‑139 (MQ=255)                                                                                                                    agaCGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGa                                                           >  2:146136/1‑99 (MQ=255)                                                                                                                    agaCGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGa                                                           <  1:146136/99‑1 (MQ=255)                                                                                                                       cGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGt                                                                                                    <  1:145424/55‑1 (MQ=255)                                                                                                                       cGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGt                                                                                                    >  2:145424/1‑55 (MQ=255)                                                                                                                       cGATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTCTCTCCACCCTGCCCTCGGCGGGCGTTGGAGCGCTGTTGgc                >  1:567307/1‑139 (MQ=255)                                                                                                                        gATGAACTCGCAGGTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTCTCTCCACCCTGCCCTCGGCGGGCGTTGGAGCGCTGTTGgcg               >  1:157208/1‑139 (MQ=255)                                                                                                                                     gTGGTCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTCTCTCCACCCTGCCCTCGGCGGGCGTTGGAGCGCTGTTGGCGCTGGAGCTGTTCa  <  1:336228/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTCTCTCCACCCTGCCCTCGGCGGGCGTTGGAGCGCTGTTGGCGCTGGAGCTGTTCA  >  NZ_CP009273/2154291‑2154561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑