Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,243,084	A→G	L5P (CTC→CCC)	yeiE ←	DNA‑binding transcriptional regulator YeiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,243,084	0	A	G	100.0%	76.3 / NA	22	L5P (CTC→CCC)	yeiE	DNA‑binding transcriptional regulator YeiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

AAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGA  >  NZ_CP009273/2242948‑2243188                                                                                                                                         |                                                                                                         aaaCAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCTCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGgggg                                                                                                        <  1:49156/139‑1 (MQ=255) aaaCAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGgggg                                                                                                        <  1:183693/139‑1 (MQ=255) aaaCAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGgggg                                                                                                        <  2:56791/139‑1 (MQ=255)  aaCAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCgggggg                                                                                                       >  2:52059/1‑139 (MQ=255)    cAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg                                                                                                     <  2:485368/139‑1 (MQ=255)       ttGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGAtg                                                                                                  >  1:183902/1‑139 (MQ=255)       ttGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGAtg                                                                                                  >  2:352968/1‑139 (MQ=255)         gCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg                                                                                                     >  2:523447/1‑134 (MQ=255)         gCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg                                                                                                     <  1:523447/134‑1 (MQ=255)              ccAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAg                                                                                           <  1:580940/139‑1 (MQ=255)              ccAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAg                                                                                           <  2:219242/139‑1 (MQ=255)                      cccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCtt                                                                                     <  2:183902/137‑1 (MQ=255)                      cccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAc                                                                                   <  2:586659/139‑1 (MQ=255)                      cccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAc                                                                                   <  2:564708/139‑1 (MQ=255)                      cccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTAc                                                                                   <  1:76102/139‑1 (MQ=255)                                   gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGat                                                                      >  1:521735/1‑139 (MQ=255)                                             cgcTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATaaa                                                            >  2:408659/1‑139 (MQ=255)                                                              gACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGgggg                                                                                                        >  2:219768/1‑78 (MQ=255)                                                              gACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGgggg                                                                                                        <  1:219768/78‑1 (MQ=255)                                                              gACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAattttat                                           >  2:525208/1‑139 (MQ=255)                                                                          aTCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGt                               <  1:52059/139‑1 (MQ=255)                                                                                    ccTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAg                                                                                           <  2:24754/69‑1 (MQ=255)                                                                                    ccTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAg                                                                                           >  1:24754/1‑69 (MQ=255)                                                                                    ccTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAAc                     >  2:302304/1‑139 (MQ=255)                                                                                             ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGccc            >  2:507525/1‑139 (MQ=255)                                                                                                    aCTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCa                                                                                              >  1:550968/1‑50 (MQ=255)                                                                                                    aCTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCa                                                                                              <  2:550968/50‑1 (MQ=255)                                                                                                        ttCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGga  <  1:525208/138‑1 (MQ=255)                                                                                                                                         |                                                                                                         AAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGA  >  NZ_CP009273/2242948‑2243188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑