Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,247,045	C→T	A194T (GCA→ACA)	nupX ←	NupC/NupG family nucleoside CNT transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,247,045	0	C	T	100.0%	76.0 / NA	21	A194T (GCA→ACA)	nupX	NupC/NupG family nucleoside CNT transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/9);  total (12/9)

TCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAG  >  NZ_CP009273/2246912‑2247171                                                                                                                                      |                                                                                                                               tCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTgtggcg                                                                                                                            <  2:367322/138‑1 (MQ=255)                      aaGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAg                                                                                                      <  1:478993/139‑1 (MQ=255)                      aaGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAg                                                                                                      <  1:592221/139‑1 (MQ=255)                          aaaCCTGCGAAGATTCCGTTGCTGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAg                                                                                                      >  2:621408/1‑135 (MQ=255)                          aaaCCTGCGAAGATTCCGTTGCTGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAg                                                                                                      <  1:621408/135‑1 (MQ=255)                           aaCCTGCGGAGATTCCGTTGCCGAGCTTAACAGGCGGGCAAACAAGATCCCGCAAGGGAACGCCATTACTGATTCCGCCAGCAGATATTCCACACGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATc                                                                                                 >  2:125238/1‑139 (MQ=255)                             ccTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATc                                                                                                 >  2:489943/1‑137 (MQ=255)                                        tCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAg                                                                                                      >  2:129944/1‑121 (MQ=255)                                        tCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAg                                                                                                      <  1:129944/121‑1 (MQ=255)                                                               ggCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCa                                                             >  2:1951/1‑139 (MQ=255)                                                                  aaaCAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTg                                                          <  1:333400/139‑1 (MQ=255)                                                                          tCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGAtt                                                     >  2:460607/1‑136 (MQ=255)                                                                            ccGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAc                                                <  1:460607/139‑1 (MQ=255)                                                                                        gCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAgg                                    >  2:600212/1‑139 (MQ=255)                                                                                                   tgCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGAtt                         >  2:576712/1‑139 (MQ=255)                                                                                                    gccgccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCAtt                                                           <  1:575558/104‑1 (MQ=255)                                                                                                    gccgccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCAtt                                                           >  2:575558/1‑104 (MQ=255)                                                                                                    gccgccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTg                        >  1:422252/1‑139 (MQ=255)                                                                                                    gccgccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTg                        >  2:481724/1‑139 (MQ=255)                                                                                                    gccgccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTg                        >  2:508731/1‑139 (MQ=255)                                                                                                                       caGGCACGCCCAGTGTGGCGTAACAAATCATTGTCGAACCAGCAATCGAGGCCATGCGACTACAAATCGGTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGTGGGAATTTCGTTTTGccc     <  1:125238/139‑1 (MQ=255)                                                                                                                          gCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAg  <  1:508731/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                               TCGGTGAAAGAGAGGTTATTAAAGGAAACCTGCGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAG  >  NZ_CP009273/2246912‑2247171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑