| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NZ_CP009273 | = 322269 | 38 (2.480) | 3 (0.210) | 3/238 | 1.3 | 8.9% | coding (690/1671 nt) | betA | choline dehydrogenase |
| ? | NZ_CP009273 | = 322352 | 26 (1.840) | coding (607/1671 nt) | betA | choline dehydrogenase | |||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
CCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGG > NZ_CP009273/322141‑322403 | ccGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCa > 1:253754/1‑138 (MQ=255) gAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGAtgt > 1:138837/1‑139 (MQ=255) cGTTGCATGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCCCCGCGCGTTTGCCGTCAAAAATGATGTGAt < 2:106320/139‑1 (MQ=255) ttGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGAGTTTGCCGTCAAAAATGATGTGATCg > 1:84589/1‑139 (MQ=255) tGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt > 2:67341/1‑136 (MQ=255) tGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt < 1:67341/136‑1 (MQ=255) cAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAGCAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGt < 1:69209/138‑1 (MQ=255) cAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGt < 1:133639/138‑1 (MQ=255) ggATCTGCGGTGAGGCAATCGCGGCTGCACATAACAGCACTTCTGTGTTGGCCGTTGCGCGGGTTTGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCAt < 2:30710/139‑1 (MQ=255) cGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGc < 2:195412/135‑1 (MQ=255) cGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGt > 1:146230/1‑137 (MQ=255) aaCAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGAcgc > 2:201808/1‑138 (MQ=255) aGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGAtt > 1:54579/1‑139 (MQ=255) cACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTg > 1:194458/1‑139 (MQ=255) ttcttTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGt > 1:119203/1‑117 (MQ=255) ttcttTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAAGGATGTGATCGGTCATAGCGTGAGTACGAATGGt < 2:119203/117‑1 (MQ=255) cttTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt > 2:36093/1‑90 (MQ=255) cttTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt < 1:36093/90‑1 (MQ=255) cttTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCt > 1:176275/1‑138 (MQ=255) gCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGc < 2:107209/92‑1 (MQ=255) gCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGc > 1:107209/1‑92 (MQ=255) cGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctctgaccgatcacatcatttttgacgg > 1:258001‑M1/1‑58 (MQ=255) cGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAg > 2:145134/1‑139 (MQ=255) cgGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCacgcg > 2:85192‑M2/1‑134 (MQ=255) cgGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCacgcg > 2:138837‑M2/1‑134 (MQ=255) cgGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGAGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCacgcg > 2:84589‑M2/1‑134 (MQ=255) gggTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCacgcgc > 1:145134‑M2/1‑133 (MQ=255) tGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGAt > 2:273708/1‑119 (MQ=255) tGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGAt < 1:273708/119‑1 (MQ=255) tGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcattt > 2:152029‑M1/1‑38 (MQ=255) tGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcattt < 1:152029‑M1/111‑74 (MQ=255) tGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt < 1:66396/60‑1 (MQ=255) tGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt > 2:66396/1‑60 (MQ=255) tGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTCCGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGgcgc > 2:70920/1‑138 (MQ=255) tGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCAAGCGCGGTGCTGGcgcg > 2:133816/1‑139 (MQ=255) ctttCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGa < 2:180259/135‑1 (MQ=255) cTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGa < 1:133816/138‑1 (MQ=255) cTGGCGCCTTCCAGCCAGTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGa < 1:85793/138‑1 (MQ=255) gCCTTCCAGCCATTCGACGCCCACCGGGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGccc < 1:247782/139‑1 (MQ=255) ttCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt > 2:10288/1‑50 (MQ=255) ttCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGAt < 1:10288/50‑1 (MQ=255) ttCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCacgcg < 1:141233‑M2/116‑6 (MQ=255) ttCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCacgcg > 2:141233‑M2/1‑111 (MQ=255) ccAGTCGACGCCCGCCGCGCGTTTGACGTCAAAGATGATGGGATCGGTCATAGCGCGAGTGCGGAGGGTCAGGTTAGGACGCGATGTGGCCTGATCGAGATAGCCACGCGCGGTGCCGGCGCGACGGCCCTGCGGCGTg < 2:167344/139‑1 (MQ=255) cATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGa < 1:58188/139‑1 (MQ=255) ttCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACg > 1:193100/1‑139 (MQ=255) gcgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcatttttgacggcaaacgcgcggtgggcgtcgaatggctggaaggcgacagcaccatcccaaccc > 1:145134‑M1/1‑6 (MQ=255) cgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcatttttgacggcaaactcgcggtgggcgtcgaatggctggaaggcgacagcaccatcccaacccg > 2:84589‑M1/1‑5 (MQ=255) cgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcatttttgacggcaaacgcgcggtgggcgtcgaatggctggaaggcgacagcaccatcccaacccg > 2:138837‑M1/1‑5 (MQ=255) cgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcatttttgacggcaaacgcgcggtgggcgtcgaatggctggaaggcgacagcaccatcccaacccg > 2:85192‑M1/1‑5 (MQ=255) cgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcatttttgacggcaaacgcgcggtgggcgtcgaatggctggaa < 1:141233‑M1/116‑112 (MQ=255) cgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgatcacatcatttttgacggcaaacgcgcggtgggcgtcgaatggctggaa > 2:141233‑M1/1‑5 (MQ=255) cgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacg < 2:66043‑M1/57‑53 (MQ=255) cgcgTggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacg > 1:66043‑M1/1‑5 (MQ=255) cgcgTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCg > 1:183566/1‑138 (MQ=255) tggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgcccgat > 1:240162‑M1/1‑1 (MQ=255) tggctatctcgatcaggccaaatcgcgtcctaacctgaccattcgtactcacgctatgaccgat < 2:240162‑M1/64‑64 (MQ=255) | CCGACGCCGGAGCGTTGCAGGATCTGCGGTGAGGCAATCGCGCCTGCACATAACAGCACTTCTTTGTTGGCCGTTGCGCGGGTTGGGATGGTGCTGTCGCCTTCCAGCCATTCGACGCCCACCGCGCGTTTGCCGTCAAAAATGATGTGATCGGTCATAGCGTGAGTACGAATGGTCAGGTTAGGACGCGATTTGGCCTGATCGAGATAGCCACGCGCGGTGCTGGCGCGACGGCCCTGCGGCGTGACGGTGCGATCCATCGG > NZ_CP009273/322141‑322403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |