| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 262,051 | 0 | T | G | 58.3% | 4.5 / 10.8 | 12 | T72P (ACC→CCC) | BW25113_RS25425 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAACCT‑GCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATC‑CTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTT > NZ_CP009273/261919‑262180 | tCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGACGT‑a > 2:228256/1‑138 (MQ=255) tCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGCGG‑AAAAACC‑GCAAGGTgg > 2:1924/1‑139 (MQ=255) cAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGAAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGG‑AAAACCTGGCAAGgggga > 1:192289/1‑138 (MQ=255) aTTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAGGTGAAAACCT‑CCACGG‑GGGGGCGGCGAAGGAGGCAACCTGTGAGCActct > 2:247514/1‑139 (MQ=255) atcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAACCT‑GCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGc < 1:1924/139‑1 (MQ=255) ggACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAAGG‑AAAAACC‑CCAAAGGGGGGCCCGGTAAATAGTGAACCCGTGAGCAATCTTTGCTGACaccatc > 2:134854/1‑139 (MQ=255) tagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAACCT‑GCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATC‑CTGGTGGCTGACCACATCACTGAAAcgcg < 2:30622/139‑1 (MQ=255) agtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGCGA‑AAAACCA‑ACAACGGGGGAACGGGAAAATAGTCAAAACGGGACCAATCTTCGCCGGCAACAAC‑CCGGAGGGCGACCACAACACCAACACGCgg > 2:243893/1‑139 (MQ=255) aCTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGCGG‑AAAAAAC‑GCAAGGTGGGGCCGGAGAAGGAGTCAACCTGTGACCAATCTTCGCTGACAACATCACTGG‑GGGGGACCAAATCACTGAAACACGgggggggg > 2:254154/1‑137 (MQ=255) tCGGGCGACTGGCGGGTACGT‑AAACCCT‑GCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTGCGCTGACATCATC‑CTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGa < 2:16344/139‑1 (MQ=255) gcGGGCGACTGGCGGGTACGT‑AAAACCT‑GCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATC‑CTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGa < 2:30536/138‑1 (MQ=255) aCTGGCGGGTACGT‑AAAACCT‑GCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATC‑CTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCtt < 1:118478/139‑1 (MQ=255) | TCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGT‑AAAACCT‑GCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATC‑CTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTT > NZ_CP009273/261919‑262180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |