| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,362 | 0 | C | T | 56.2% | ‑6.5 / 11.2 | 16 | intergenic (+52/‑51) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base T (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.49e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGA‑C‑ATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGA > NZ_CP009273/2380228‑2380495 | gTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACC‑AGGCA‑ATTTTATTTTTCCTc > 2:245079/1‑139 (MQ=255) aTACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGA‑C‑ATGCACATTTTATTTTCCCTCAt < 1:60155/138‑1 (MQ=255) aTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGG‑C‑ATGAACAATTTATTTTTCCTCATCTGTTTTTAGATGc > 1:18609/1‑139 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGA‑C‑ATGTACATTTTATTTTTCCTCCTCTGTTTTAAGAAGCGAAAAATG‑AAAATCAAAGGAGGGAAAATAAATggg > 1:203484/1‑139 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCTGAAAAAAAGA‑A‑ATGAACATTTTATTTTTTCTCCTCTGTTTTTAAATGCGAAACATG‑AAAATTAACCGTGGAAAAATAAATtgg > 2:15896/1‑138 (MQ=255) ttATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAACGA‑C‑ATGCACAATTTTTTTTTCCTCATCTGTTTTTAAATGCGAAACAAG‑AAAATTAAAGGGGGGAAAATAAATGGGGAt > 1:3265/1‑139 (MQ=255) ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCAAAAAAAAAGA‑CAAAAAAAATTTAATTTTTCTTCA‑CTGGTTTTAAAAGCGAAAAAAGAAAATTAAAATGGGGAAAAA‑AAATGGGAAAGAtttgg > 2:234132/1‑135 (MQ=255) gTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAACA‑C‑AAGCAAATTTTATTTTTTCTCATCTGGTTTAAGATGCGAAAAAAT‑AAAATCAACGGAGGAAAAATAAATGGGGATGGTTGGCAATTTTTAAGAAAt > 2:179095/1‑139 (MQ=255) gTAAATAATAAAAATTTCTCGAGGCGGATCCAGAAAAAAAGC‑G‑AATCAAATTTTATTTTTTCTTATCTTTTTTTAGAAGCGGAAAAAG‑AAATTTAACGGCGAAAAAATAAATGGGAATGTTTGGCTTTTTTTCAGaaaa > 1:244018/1‑138 (MQ=255) taaAAATTTCTCGAGGCGGGTCCCGAAAAAGCGA‑C‑AAGTACATTTTATTTTTCCTCATCTGGTTTTAGATGCGGAACATG‑AAAATTAACGGAGGAAAAATAAATGGGAATGATTGGGTTTTTTGCAGAAAATGAcaca > 1:211351/1‑138 (MQ=255) gTCCAGAAAAAGCGA‑C‑ATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCt < 2:211351/139‑1 (MQ=255) aGCGA‑C‑ATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCaa > 1:253894/1‑59 (MQ=255) aGCGA‑C‑ATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCaa < 2:253894/59‑1 (MQ=255) gCGA‑C‑ATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACt > 2:76299/1‑138 (MQ=255) ttattttCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGAt < 1:138292/138‑1 (MQ=255) ttttCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGa < 2:18609/138‑1 (MQ=255) | GTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGA‑C‑ATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATG‑AAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGA > NZ_CP009273/2380228‑2380495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |