Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,588	T→C	L71P (CTG→CCG)	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,588	0	T	C	100.0%	45.9 / NA	14	L71P (CTG→CCG)	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

GATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGT  >  NZ_CP009273/1613452‑1613720                                                                                                                                         |                                                                                                                                     gaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGa                                                                                                                                    >  1:144832/1‑139 (MQ=255)     gCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCg                                                                                                                                >  2:162460/1‑139 (MQ=255)                 aGTATTTGTCTCCGCTGGATATTACCTCGGCACAG‑TTAAGGTGCTCCGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGTCCTGGGAGCACCGACCCGTATGCTGGATCGc                                                                                                                   <  1:268818/139‑1 (MQ=255)                             cGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTa                                                                                                         >  1:191402/1‑138 (MQ=255)                                            cgGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAg                                                                                         >  1:105/1‑139 (MQ=255)                                                              tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGAccc                                                                                                                                 <  1:11934/81‑1 (MQ=255)                                                              tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGAccc                                                                                                                                 >  2:11934/1‑81 (MQ=255)                                                              tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGt                                                                                                              <  1:155318/100‑1 (MQ=255)                                                              tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGt                                                                                                              >  2:155318/1‑100 (MQ=255)                                                                                                 gTTGAACTGCAAAAGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGCCTGTAAAGGCTGGGCGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGGACTGGCAAAACTTATCACcggcg                                    >  2:202532/1‑139 (MQ=255)                                                                                                          aaaaaGGTATTGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgcggc                             <  2:43419/137‑1 (MQ=255)                                                                                                                   ttGTCGGTCGACCTGGGAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGaa                   <  1:162460/138‑1 (MQ=255)                                                                                                                                   gAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt  <  2:144832/139‑1 (MQ=255)                                                                                                                                   gAGCACCGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGt  <  2:206196/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGT  >  NZ_CP009273/1613452‑1613720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑