Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,631,170	G→C	A57P (GCG→CCG)	pitA →	inorganic phosphate transporter PitA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,631,170	0	G	C	100.0%	100.3 / NA	27	A57P (GCG→CCG)	pitA	inorganic phosphate transporter PitA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/14);  total (13/14)

ATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGATCGTCTCATGGCCTTGCCATGGTGTT  >  NZ_CP009273/3631046‑3631294                                                                                                                             |                                                                                                                             attattGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCaa                                                                                                                 <  2:227529/138‑1 (MQ=255)    attGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACttt                                                                                                             >  1:107112/1‑139 (MQ=255)    attGCTTGCACTGGCTTTTGAGCTGTTCTACGAAGCCATCAATGGTTTCCATGAGACAGCCAACGACGTGGAAACCGTTATCTATACATGCGCGATGCGATCTCAGATCGCCGTGGTAATGCCGGCGGTATTCAACttt                                                                                                             >  2:117079/1‑139 (MQ=255)     ttgcttgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACtttt                                                                                                            >  1:206325/1‑139 (MQ=255)         ttgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTggg                                                                                                        >  2:271494/1‑139 (MQ=255)          tgcACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGccggcg                                                                                                                         <  2:272773/121‑1 (MQ=255)          tgcACTGGCTTTTGTGCTGTTCTACGAAGCCATAAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGacggcg                                                                                                                         >  1:272773/1‑121 (MQ=255)                     ttGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTg                                                                                              <  2:206325/137‑1 (MQ=255)                          cTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTc                                                                                       <  1:231140/139‑1 (MQ=255)                             ttCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCt                                                                                      >  1:162134/1‑137 (MQ=255)                                            aaTGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCa                                                                     <  1:7830/139‑1 (MQ=255)                                             aTGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCAt                                                                    <  1:129343/139‑1 (MQ=255)                                                   tCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGGGGTCTGAGGGTTGCCTATGCCCTTTTGCa                                                              >  2:41532/1‑139 (MQ=255)                                                           acaGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATAtgctgc                                                      >  2:198517/1‑139 (MQ=255)                                                                      cGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATctg                                           <  1:53716/139‑1 (MQ=255)                                                                           cAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTgctgct                                      <  1:116342/139‑1 (MQ=255)                                                                                        taCCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGAt                           <  2:188349/137‑1 (MQ=255)                                                                                         aCCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGGTCTGCTGCTTAATATGGGATCGt                        >  2:53202/1‑139 (MQ=255)                                                                                         aCCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATctgctg                                        >  1:55785/1‑123 (MQ=255)                                                                                         aCCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATctgctg                                        <  2:55785/123‑1 (MQ=255)                                                                                              cgcgATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCACCTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGTTGCCGACGGATCTGCTGCTTAATATGGGGTCGTCTCAt                   >  2:95490/1‑139 (MQ=255)                                                                                              cgcgATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGATCGTCTCAt                   >  2:177576/1‑139 (MQ=255)                                                                                              cgcgATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGGGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGATCGTCTCAt                   >  2:14151/1‑139 (MQ=255)                                                                                                cgATGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGATCGTCTCATgg                 <  1:100223/139‑1 (MQ=255)                                                                                                  aTGCGTTCTCAGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGATCGTCTCATGGcc               <  2:162134/139‑1 (MQ=255)                                                                                                            aGCTCGCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGTGATCGTCTCATGGCCTTGCCATgg      <  1:272178/138‑1 (MQ=255)                                                                                                                 gCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATg                                                                        <  1:96051/67‑1 (MQ=255)                                                                                                                 gCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATg                                                                        >  2:96051/1‑67 (MQ=255)                                                                                                                 gCCGTGGTTATGCCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGATCGTCTCATGGCCTTGCCATGGTGtt  <  1:198517/137‑1 (MQ=255)                                                                                                                             |                                                                                                                             ATTATTGCTTGCACTGGCTTTTGTGCTGTTCTACGAAGCCATCAATGGTTTCCATGACACAGCCAACGCCGTGGCAACCGTTATCTATACCCGCGCGATGCGTTCTCAGCTCGCCGTGGTTATGGCGGCGGTATTCAACTTTTTGGGTGTTTTGCTGGGTGGTCTGAGTGTTGCCTATGCCATTGTGCATATGCTGCCGACGGATCTGCTGCTTAATATGGGATCGTCTCATGGCCTTGCCATGGTGTT  >  NZ_CP009273/3631046‑3631294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑