| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 15,410 | 0 | T | G | 57.1% | 7.0 / 17.1 | 14 | intergenic (+112/‑35) | dnaJ/BW25113_RS00080 | molecular chaperone DnaJ/IS4‑like element IS421 family transposase |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA > NZ_CP009273/15275‑15524 | ttttttGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTtgt < 2:145110/139‑1 (MQ=255) aCCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGat < 1:450341/139‑1 (MQ=255) cTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAGCGCTAACTTAAGGGGTGTGGTGTTATGCCTGATTTGAt > 2:283517/1‑139 (MQ=255) cAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGcc < 1:454405/139‑1 (MQ=255) ggCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGACACTCCCCTAAGCGCTAAATTTAAGGGTGTGGTATTACGCCTGATATGATTTAAAGTGCCGAt > 1:86572/2‑138 (MQ=37) ggTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCATAAGCGCTAACTTAAAGGGTGTGGGATTACGCCTGGTATGATTTAACGTGCCGATGAATTACTCTCACGATAACAGGTCAGCAATTCTg > 1:6243/1‑139 (MQ=18) tGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc < 2:124615/139‑1 (MQ=255) cGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGccc < 2:284058/139‑1 (MQ=255) atGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCATAAACGCTAACTTTAAGGGTGTGGGATTACGCCCGATATGATTTTACGTGCCGGTGAATTAATCTCACGATAAATGGTCAGCAATTCTTGCCCCTATTTGtt > 1:609358/1‑137 (MQ=255) atGCGAGCACCTGTAAAGTGGCGGGGGTCAATCCCATAAGAGATAACTTAAGGGGTGGGGGAAGAAGCCTGATATGATTTAAAGTGGCGATGAATTAATCTCACGATAACTGGGCAACAAATTTGGCGCataagtgta > 1:135345/1‑132 (MQ=255) gCGGGGGTCACTCCCCTAAACGCTAACTTAAAGGGTGTGGGATTATGCCTGATATGATTTTAAGTGCCGATGAATTACTCTCACGATAAATGGTCAGCAATTTTGGCCCATATTGGTAAACCCGAAGAAATGGATACtt > 2:402264/1‑139 (MQ=11) gCGGGGGTCACTCCCCTAAACGCTAAATTAAGGGGTGTGGTATTACGCCCGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACAGGTCAGCAATTTTGGCCCCTATTTGTAAGCCCGAAGAACTGGATACtt > 2:359288/1‑139 (MQ=11) ggggTCACTCCCATAAGCGCTTACTTAAGGGGTGTGGGATTACGCCTGATATGATTTTAAGTGCCGATGAATTAATCTCACGATAAATGGTCAGCAATTCTTGCCCATATTGGGAAACCCGAAGAACTGGGAACTTCgg > 2:184239/1‑139 (MQ=11) cTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTaa > 1:282829/1‑139 (MQ=11) | TTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAA > NZ_CP009273/15275‑15524 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |