| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 497,031 | 0 | A | C | 52.9% | 17.0 / 23.3 | 17 | V555G (GTG→GGG) | ybaL | Kef family K(+) transporter |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base C (0/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAA > NZ_CP009273/496924‑497163 | cgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTc > 2:250827/1‑139 (MQ=255)cgcATCGGGCTTTTGGTTCTCTTTGCCTGTTGCGACGCTGCCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTc < 2:418250/139‑1 (MQ=37) ttGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTATTTTTTTACATCGGGCACCAACCGTTACCCCCGTCACAACTTCACCGGCCGGGGGGGTTTCAAGCATTTCCAGAATGGAACg > 1:110067/1‑139 (MQ=21) ttGGCTCTCGATCCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTCCACCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTa < 2:174570/137‑1 (MQ=38) tcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑tt > 1:483789/1‑139 (MQ=255) cTTATCATGCCTACATATTTTTCATATTTTACACCCGGCAACCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGAtt < 2:356152/138‑1 (MQ=255) aTGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGcacc < 1:427757/138‑1 (MQ=255) ccaatttttttCATATTTTCCATCCGGCACCCACCGTTTCCCCCGCCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCATTTCCAGCATGGTACGGGCGATTTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt < 2:110067/133‑1 (MQ=255) cATATTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCg < 1:343077/138‑1 (MQ=255) cATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg > 2:589944/1‑139 (MQ=255) cacccGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa < 1:250827/135‑1 (MQ=255) cATCCGGCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa < 1:35343/138‑1 (MQ=255) cATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa < 2:483789/138‑1 (MQ=255) tttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGcgcg > 1:596208/1‑139 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcg < 1:509527/130‑1 (MQ=255) cGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcg > 2:509527/1‑130 (MQ=255) gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataa > 2:338118/1‑139 (MQ=255) | CGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGA‑TTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAA > NZ_CP009273/496924‑497163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |